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目的以苯并(a)芘诱导小鼠前胃癌模型,研究番茄红素对前胃癌小鼠氧化损伤的保护作用。方法将120只昆明种小鼠随机分为4组:正常对照组、损伤组[苯并(a)芘组]、番茄红素高剂量组[20 mg/kg+苯并(a)芘]、番茄红素低剂量组[5 mg/kg+苯并(a)芘]。番茄红素组与损伤组给予苯并(a)芘,建立前胃癌模型,观察不同浓度的番茄红素对小鼠前胃癌形成的作用。24周后,观察小鼠前胃癌形成情况,检测血清及肝匀浆中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)的含量,同时检测淋巴细胞DNA氧化损伤情况。结果番茄红素具有明显的抗肿瘤作用,给予番茄红素后能降低小鼠前胃癌肿瘤直径[模型组(0.33±0.20)cm,番茄红素高剂量+苯并(a)芘组(0.12±0.04)cm],提高小鼠血清和肝匀浆中SOD活力[损伤组血清中为(47.18±3.27)U/ml,肝匀浆中为(39.83±6.63)U/mg.Pro;番茄红素高剂量组血清中为(67.27±9.38)U/ml,肝匀浆中为(54.49±4.86)U/mg.Pro]、GSH-Px活力[损伤组血清中为(170.63±15.25)U/ml,肝匀浆中为(54.60±5.19)U/mg.Pro;番茄红素高剂量组血清中为(184.22±13.81)U/ml.Pro,肝匀浆中为(66.78±11.97)U/mg.Pro],降低MDA含量[模型组血清中为(11.08±1.82)nmol/mg,肝匀浆中为(12.47±2.62)nmol/mg.Pro;番茄红素高剂量组血清(7.63±0.85)nmol/mg,肝匀浆(8.28±1.74)nmol/mg.Pro]和减少淋巴细胞DNA的氧化损伤的作用,差异均有统计学意义(P<0.05)。结论番茄红素对苯并(a)芘诱导的小鼠前胃癌具有一定的抑制作用,对细胞DNA氧化损伤具有明显的保护作用。
OBJECTIVE: To study the protective effect of lycopene on oxidative damage in mice with precancerous gastric cancer induced by benzo (a) pyrene. Methods 120 Kunming mice were randomly divided into 4 groups: normal control group, injured group (benzo (a) pyrene group, high-dose lycopene group [20 mg / kg and benzo [a] pyrene] Low-dose red group [5 mg / kg + benzo (a) pyrene]. Benzo (a) pyrene was given to lycopene group and injury group, and the model of precancerous gastric cancer was established. The effect of different concentrations of lycopene on the formation of mouse gastric cancer was observed. Twenty-four weeks later, the formation of gastric precancerous mice was observed. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) Content, simultaneous detection of lymphocyte DNA oxidative damage. Results Lycopene had a significant antitumor effect. The lycopene could decrease the tumor diameter of mice with gastric cancer [the model group (0.33 ± 0.20) cm, the high dose of lycopene + (a) pyrene group (0.12 ± 0.04) cm], and increased SOD activity in serum and liver homogenate of mice [(47.18 ± 3.27) U / ml in serum and (39.83 ± 6.63) U / mg.Pro in liver homogenate) (67.27 ± 9.38 U / ml in the high-dose group and (54.49 ± 4.86) U / mg.Pro in the liver homogenate). The activity of GSH-Px in the serum of the high dose group was (170.63 ± 15.25) U / ml (54.60 ± 5.19) U / mg.Pro in the liver homogenate, (184.22 ± 13.81) U / ml.Pro in the high-dose lycopene group and (66.78 ± 11.97) U / mg in the liver homogenate (11.08 ± 1.82) nmol / mg in the serum of the model group and (12.47 ± 2.62) nmol / mg.Pro in the liver homogenate, and 7.63 ± 0.85 in the high-dose lycopene group nmol / mg, liver homogenate (8.28 ± 1.74) nmol / mg.Pro], and the effect of reducing the oxidative damage of DNA in lymphocytes were statistically significant (P <0.05). Conclusions Lycopene has certain inhibitory effect on benzo (a) pyrene-induced gastric cancer in mice, which has a significant protective effect on DNA oxidative damage.