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在美国,一个新的快速方法——玫瑰红平板试验已用于诊断牛布氏杆菌病,本研究的目的是把玫瑰红平板试验与其他血清学试验相比较,以判别其特异性和敏感性。共采集440份人的血清,其中51例为急性布氏杆菌病患者,173例为慢性患者,99例接种过布氏杆菌19—BA 株活疫苗,117例来自在牛布氏杆菌病疫源地生活和工作的人员。全部血清均进行玫瑰红平板凝集试验、Hud-dleson 试验、试管凝集试验,被动血凝试验和 Coombs 试验,并于进行血清试验前检测血清免疫球蛋白的成分,特别是 IgG(经盐酸半胱氨酸处理)。本试验在平板上进行,取等量(0.03毫升)血清和抗原置于平
In the United States, a new rapid method, the Rose Bengal test, has been used to diagnose brucellosis. The purpose of this study was to compare the Rose Bengal test with other serological tests to determine its specificity and sensitivity . A total of 440 human sera were collected, of which 51 were acute brucellosis patients, 173 were chronic patients, 99 were inoculated with Brucella 19-BA live vaccine, and 117 were from bovine brucellosis outbreaks People who live and work. All sera were tested for rosiglitazone agglutination test, Hud-dleson test, tube agglutination test, passive hemagglutination test and Coombs test, and the serum immunoglobulin components were tested before serum test, especially IgG (cysteamine hydrochloride Acid treatment). The test on the plate, take the same amount (0.03 ml) of serum and antigen placed in the flat