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利用碱抽提法从串珠镰刀菌 ( Fusarium moniliforme)菌丝细胞壁得到多糖粗提物。该多糖提取物可以激活寄生于串珠镰刀菌上的活体寄生真菌纤细齿梗孢 ( Olpitrichum tenellum)的孢子萌发 ,多糖浓度为 0 .2 mg/m L时 ,萌发率达 80 .5 %。纤细齿梗孢的孢子仅在存在该多糖提取物的条件下才能萌发 ,在马铃薯 -葡萄糖或葡萄糖 -酵母液体培养基上不能萌发 ,一些糖、氨基酸、无机盐、维生素不能促使其萌发 ,光也不影响萌发。纤细齿梗孢孢子萌发最适 p H是 7.0 ,最适温度 2 5℃。结果表明 ,串珠镰刀菌菌丝细胞壁多糖可能在激活纤细齿梗孢孢子萌发中起重要作用。
A crude polysaccharide extract was obtained from the mycelial cell wall of Fusarium moniliforme by alkaline extraction. The polysaccharide extract can activate the spore germination of the parasitic fungus Olipitrichum tenellum parasitized on Fusarium moniliforme, with a germination rate of 80.5% when the polysaccharide concentration is 0.2 mg / mL. Spores of Trichophylet sp. Germinate only in the presence of the polysaccharide extract, and can not germinate on potato-dextrose or dextrose-yeast broth. Some sugars, amino acids, inorganic salts and vitamins can not induce germination. Does not affect the germination. Stemospore spore germination optimum p H is 7.0, the optimum temperature of 25 ℃. The results showed that Fusarium moniliforme cell wall polysaccharides may play an important role in the activation of spore germination.