论文部分内容阅读
目的分析丙型病毒性肝炎(丙肝)病毒(HCV)的基因型与病毒载量及肝脏疾病进展的相关性。方法选择2010年12月至2013年4月在宁波市第二医院北郊院区就诊的慢性HCV感染者,分别采用反转录-聚合酶链反应(RT-PCR)法和基因芯片法检测HCV RNA和HCV基因型。结果 107例标本中,共检出7种亚型,其中1a型4例(3.74%),1b型52例(48.60%),2a型15例(14.02%),3a型10例(9.35%),3b型9例(8.41%),6型16例(14.95%),1b+2a混合型1例(0.93%);男女慢性HCV感染均以1型为主要基因型,但男性6型的构成比高于女性(χ2=4.336,P=0.049);各年龄组间HCV基因型的构成及各基因型的年龄构成差异均无统计学意义;6型的病毒载量最高,其次为1型,2、3型的病毒载量明显低于6型和1型(P<0.01);终末期肝病患者和伴存免疫缺陷患者的病毒载量均高于慢性丙肝患者,且终末期肝病患者以基因1型(主要为1b)为主(P=0.016)。结论本地区HCV基因型同样以1b型为主,且1b型感染者的病毒载量较高,疾病进展较快;6型的感染率较高,应重视对基因6型HCV感染者的研究。
Objective To analyze the relationship between genotypes of hepatitis C virus (HCV) and viral load and the progress of liver diseases. Methods Chronic HCV infection in the northern outskirts of Ningbo No.2 Hospital from December 2010 to April 2013 were detected by reverse transcription polymerase chain reaction (RT-PCR) and microarray RNA and HCV genotypes. Results Of the 107 specimens, 7 subtypes were identified, of which 4 cases (3.74%) were type 1a, 52 (48.60%) were type 1b, 15 (14.02%) were type 2a and 10 (9.35% , 9 (8.41%) in type 3b, 16 (14.95%) in type 6, and 1 case (1b + 2a) in 1c (+ 0.93%). Chronic HCV infection in both genotypes was mainly genotype 1, (Χ2 = 4.336, P = 0.049). There were no significant differences in the genotypes of HCV genotypes and genotypes among different age groups. Type 6 had the highest viral load, followed by type 1, The viral load of type 2 and 3 was significantly lower than that of type 6 and 1 (P <0.01). The viral load of patients with end-stage liver disease and concomitant immune deficiency were higher than that of patients with chronic hepatitis C, Type 1 (mainly 1b) (P = 0.016). Conclusion The genotypes of HCV genotype 1b are also predominant in this region, and the genotype 1b virus has a higher viral load and rapid disease progression. The genotype 6 has a higher infection rate and should be considered for genotype 6 HCV infection.