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用酵母系统筛选微生物产生的免疫抑制活性化合物，从３００多株放线菌中筛选出阳性菌株ＳＩＰＩ－９７－８４，对其代谢产物进行初步研究，用丙酮浸泡、溶媒萃取、硅胶柱层析和重结晶等方法，从其菌丝体中分离出活性成分ＳＩＰＩ－８４－１５。通过元素分析和质谱，确定其分子式为Ｃ２８Ｈ３１ＮＯ６（分子量：４７７．２１４５）；综合紫外光谱、红外光谱、１Ｈ－ＮＭＲ、１３Ｃ－ＮＭＲ等图谱数据，确定ＳＩＰＩ－８４－１５的结构为含有硝基的多烯化合物，该结构与文献报道的ｓｐｅｃｔｉｎａｂｉｌｉｎ一致，化学命名为：２－甲氧基－３，５－二甲基－６－［四氢－４－［２，４，６－三甲基－７－（４－硝基苯基）－２，４，６－庚三烯亚基］－２－呋喃基］，４Ｈ－吡喃－４－酮。
Screening of immunosuppressive compounds produced by microorganisms by yeast system, the positive strain SIPI-97-84 was screened from more than 300 actinomycetes and its metabolites were preliminarily studied. After soaking in acetone, solvent extraction and silica gel column chromatography Recrystallization and the like, the active ingredient SIPI-84-15 was isolated from its mycelium. The molecular structure of SIPI-84-15 was confirmed to be C28H31NO6 (molecular weight: 477.2145) by elemental analysis and mass spectrometry. The structure of SIPI-84-15 was confirmed to be nitro group-containing The structure of the polyene compound is consistent with the reported spectinabilin. The chemical name is 2-methoxy-3,5-dimethyl-6- [tetrahydro-4- [2,4,6-trimethyl- 7- (4-nitrophenyl) -2,4,6-heptatrienylene] -2-furanyl], 4H-pyran-4-one.