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目的探讨内皮抑制素质粒重复注射治疗小鼠肝癌的效果及其作用机制。方法内皮抑制素质粒转染BHK21,ELISA检测培养上清中内皮抑制素的含量,并用此上清作用于ECV304内皮细胞,MTT法检测内皮细胞的增殖。小鼠股部肌内接种H22细胞,反复注射内皮抑制素质粒/PVP,观察肿瘤的形成。ELISA检测血清中内皮抑制素的含量,免疫组化观察肿瘤血管密度,TUNEL检测肿瘤细胞的凋亡。结果转染上清中可以检测到分泌出的内皮抑制素,并可抑制内皮细胞的增殖,抑制率约29.2%。治疗组血清内皮抑制素水平升高,瘤内血管减少,肿瘤细胞凋亡增加,肝癌生长受抑,抑制率为56.4%。结论PVP介导的内皮抑制素基因治疗可以抑制小鼠肝癌血管形成,从而较好地抑制了肝癌的生长。
Objective To investigate the effect and mechanism of repeated injections of endostatin plasmid in the treatment of mouse liver cancer. Methods Endostatin plasmids were transfected into BHK21, and the content of endostatin in culture supernatants was detected by ELISA. ECV304 endothelial cells were treated with this supernatant, and the proliferation of endothelial cells was detected by MTT assay. H22 cells were inoculated intramuscularly in the femoral part of the mice and the endostatin plasmid / PVP was injected repeatedly to observe the formation of the tumor. The content of endostatin in serum was detected by ELISA, the density of tumor vessels was observed by immunohistochemistry, and the apoptosis of tumor cells was detected by TUNEL. Results The transfected supernatant could detect secreted endostatin and inhibit the proliferation of endothelial cells with the inhibition rate of about 29.2%. Treatment group, serum endostatin levels increased, intratumoral vascular reduction, tumor cell apoptosis increased, liver cancer growth inhibition, inhibition rate was 56.4%. Conclusion PVP-mediated endostatin gene therapy can inhibit the formation of hepatic carcinoma in mice and thus inhibit the growth of hepatocellular carcinoma.