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目的建立以HPLC法同时测定利胆排石片中木香烃内酯和去氢木香内酯含量的方法。方法色谱柱:依利特SinoChrom ODS-BP柱(4.6 mm×200 mm,5μm),流动相:乙腈-水(体积比为65∶35),流速:1.0 mL.min-1,检测波长:225 nm,柱温:室温。结果木香烃内酯和去氢木香内酯的质量浓度分别在6.160~123.200 mg.L-1和4.600~92.000 mg.L-1内与峰面积呈良好的线性关系,相关系数分别0.999 7和0.999 6;平均回收率分别为100.6%和99.9%,RSD分别为1.0%和0.9%。结论本方法可作为利胆排石片的质量控制方法之一。
Objective To establish a HPLC method for the simultaneous determination of the contents of costunolide and dehydrocostuslactone in Lidan Paishi tablets. Methods Chromatographic column: Elite Chrom ODS-BP column (4.6 mm×200 mm, 5 μm), mobile phase: acetonitrile-water (65:35 in volume), flow rate: 1.0 mL.min-1, detection wavelength: 225 nm Column temperature: room temperature. The results showed that the mass concentrations of the decoction and dehydrocostus lactone were in a good linear relationship with the peak area within 6.160~123.200 mg.L-1 and 4.600~92.000 mg.L-1, respectively. The correlation coefficient was 0.999 7 respectively. And 0.999 6; average recovery was 100.6% and 99.9%, respectively, RSD 1.0% and 0.9%. Conclusion This method can be used as one of the methods for quality control of gallbladder discs.