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目的:研究大蒜新素对巨细胞病毒(CMV)感染鼠的免疫抑制性细胞因子TGF-β1和IL-10的影响,探讨大蒜新素的抗CMV机制。方法:建立非免疫抑制性鼠巨细胞病毒(MCMV)全身播散性感染小鼠模型;双抗体夹心ELISA法检测感染后1,3,7,14,28,45,60,75,90,120d正常对照组、病毒感染组和药物治疗组小鼠脾细胞培养上清中TGF-β1和IL-10水平的时序性变化。结果:MCMV感染后,TGF-β1和IL-10分泌均显著升高,并呈现出急慢性期两次高峰的特点。大蒜新素治疗对MCMV感染鼠各时间点TGF-β1水平均有下调作用,且对其急性期分泌高峰的抑制更明显。大蒜新素治疗对IL-10的急性期分泌高峰影响不明显,却显著下调慢性期IL-10的分泌高峰。结论:大蒜新素能显著抑制MCMV感染后TGF-β1和IL-10分泌,进而增强机体细胞免疫功能而有利于机体清除MCMV病毒,这可能是大蒜新素抗CMV的另一作用机制。
OBJECTIVE: To study the effect of allitridin on immune suppressive cytokines TGF-β1 and IL-10 in mice infected with cytomegalovirus (CMV) and to investigate the mechanism of anti-CMV of allitin. METHODS: A mouse model of non-immunosuppressive murine cytomegalovirus (MCMV) systemic disseminated infection was established; double antibody sandwich ELISA was used to detect 1, 3, 7, 14, 28, 45, 60, 75, 90, 120 days after infection. Sequential changes in the levels of TGF-β1 and IL-10 in culture supernatants of splenocytes from control, virus-infected, and drug-treated mice. RESULTS: After MCMV infection, the secretion of TGF-β1 and IL-10 was significantly increased, and showed two peaks in acute and chronic phase. Allicin treatment had a down-regulation of TGF-β1 levels in MCMV-infected mice at various time points, and the inhibition of acute peak secretion was more obvious. The allicin treatment had no significant effect on the acute peak of IL-10 secretion, but it significantly down-regulated the secretion of IL-10 in the chronic phase. Conclusion: Alliin can significantly inhibit the secretion of TGF-β1 and IL-10 after MCMV infection, and then enhance the cellular immune function and facilitate the removal of MCMV virus. This may be another mechanism of action of alliin against CMV.