【摘 要】
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Objective:To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis.Methods:This systems-based interactome analysis was designed to enforce the workflow of “ASR (herb)→co
【机 构】
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Information Science and Engineering School, Lanzhou University, Lanzhou 730000, China;School of Biol
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Objective:To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis.Methods:This systems-based interactome analysis was designed to enforce the workflow of “ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis”.This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments.Results:The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK),Janus kinase 2 (JAK2),and interleukin-2-inducible T-cell kinase (ITK).The hematopoietic function of ASR was also validated by colonyforming assay performed on mice bone marrow cells.As a result,SYK,JAK2 and ITK were activated.Conclusion:This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.
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