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为研究商陆皂苷甲的生物合成途径,利用Illumina Hi Seq 4000高通量测序技术对商陆幼苗进行转录组测序,得到9.60 Gb clean data,经Trinity软件组装后获得63 957条unigenes,平均长度988.82 bp,其中24 517条unigenes(38.33%)能被Nr、Swiss-Prot、COG、KOG、Pfam、GO、KEGG等公共数据库注释。对注释得到的unigenes进行KEGG代谢通路分析,发现商陆转录组中有53个unigenes参与萜类骨架合成通路,有8个unigenes参与三萜合成通路,还有417个unigenes参与商陆其他次生代谢途径。进一步分析参与商陆皂苷甲生物合成后修饰酶相关基因,发现有130个unigenes可能具有CYP450的功能,参与商陆次生代谢产物的氧化/羟基化修饰;有46个unigenes与糖基转移酶UGT相关。商陆转录组数据的获得为研究商陆皂苷甲和其他次生代谢产物的生物合成途径奠定了基础,也为商陆药材品质的形成提供理论依据。
In order to study the biosynthetic pathway of esculentoside A, we sequenced the transcriptome of commercial seedlings using Illumina Hi Seq 4000 high-throughput sequencing technology to obtain 9.60 Gb clean data. After assembly by Trinity software, 63 957 unigenes were obtained with an average length of 988.82 bp, of which 24,517 unigenes (38.33%) were annotated by public databases such as Nr, Swiss-Prot, COG, KOG, Pfam, GO and KEGG. KEGG metabolic pathway analysis of annotated unigenes showed that 53 unigenes involved in terpenoid synthesis pathway, 8 unigenes involved in triterpene synthesis pathway and 417 unigenes participated in other secondary metabolism of pokeweed way. Further analysis of genes involved in the modification of esterase after the biosynthesis of esculentosus argenteoside revealed that 130 unigenes may have the function of CYP450 and participate in the oxidative / hydroxylation of the secondary metabolites of esculenta. 46 unigenes were associated with glycosyltransferase UGT. The accession of pokeweed transcriptome data laid the foundation for the study of the biosynthetic pathway of esculentoside A and other secondary metabolites, and provided the theoretical basis for the formation of the medicinal quality of pokeweed.