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采用一次性递增负荷跑台运动对不同浓度PM2.5染毒大鼠进行训练,通过测定含量的变化,研究运动、PM2.5染毒及24 h恢复对大鼠血清肿瘤坏死因子-α(Tumor Necrosis Factor,TNF-α)、超敏C反应蛋白(hypersensitive C-reactive protein,hs-CRP)、克拉拉细胞蛋白(Clara Cell Protein,CC16)的影响机制,选取雄性SD大鼠64只(每组8只),随机分为运动即刻组(EC)、24 h恢复组(ECR)、7.5 mg·kg-1(LPE)、15 mg·kg-1(MPE)、30 mg·kg-1(HPE)、7.5 mg·kg-1+24 h恢复组(LPER)、15 mg·kg-1PM2.5+24 h恢复组(MPER)、30 mg·kg-1+24 h恢复组(HPER).采用气管滴注法PM2.5染毒然后进行一次性递增负荷跑台进行训练,运动即刻组与恢复组分别于训练后即刻及恢复24 h后处死,提取血清后通过酶联免疫法(Enzymelinked immuno sorbent assay,ELISA)测定TNF-α、hs-CRP、CC16,并进行有关统计学检验.结果表明,与运动对照组相比,不同浓度PM2.5染毒组大鼠运动后,即刻血清TNF-α及hs-CRP水平升高,而CC16水平在染毒组运动后即刻降低,24 h后TNF-α、hs-CRP、CC16基本恢复到对照组的水平且与染毒浓度有剂量相关关系.
One-time incremental treadmill exercise was used to train rats exposed to different concentrations of PM2.5. The levels of TNF-α in serum, PM2.5 and 24 h recovery (TNF-α), hypersensitive C-reactive protein (hs-CRP) and Clara Cell Protein (CC16) 8 rats) were randomly divided into exercise group (EC), 24 h recovery group (ECR), 7.5 mg · kg -1 (LPE), 15 mg · kg -1 (MPE), 30 mg · kg -1 ), 7.5 mg · kg-1 + 24 h recovery group (LPER), 15 mg · kg-1 PM2.5 + 24 h recovery group (MPER) and 30 mg · kg-1 + Tracheal instillation PM2.5 and then a one-time incremental load treadmill training, exercise immediate group and recovery group were immediately after training and recovery 24 h after the death, serum was extracted by enzyme-linked immunosorbent assay (Enzymelinked immuno sorbent The levels of TNF-α, hs-CRP and CC16 were measured by ELISA assay.The results showed that compared with the control group, the concentrations of TNF-α And h s-CRP levels increased, while the level of CC16 decreased immediately after exercise. After 24 h, the levels of TNF-α, hs-CRP and CC16 returned to control levels and dose-dependently.