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目的:研究斑蝥素对H O- 8910 PM细胞内N F- κB(P6 5 )、Smad3蛋白及NF- κB(P6 5 ) m RN A表达的影响,探讨其抗肿瘤侵袭转移的作用机制。方法:用Western blot分析N F- κB(P6 5 )及Smad3蛋白的表达;RT- PCR分析NF- κB(P6 5 ) m RNA的表达。结果:2 5～10 0μmol/ L的斑蝥素作用于H O- 8910 PM细胞2 4 h后,明显下调N F-κB(P6 5 )及Smad3蛋白表达和NF-κB(P6 5 ) m RN A表达(P<0 .0 1) ,并呈一定的剂量效应。结论:斑蝥素抗肿瘤侵袭、转移与抑制NF- κB(P6 5 )、Smad3蛋白和NF- κB(P6 5 ) m RNA的表达有关。
Objective: To investigate the effect of cantharidin on the expression of N F-κB (P6 5), Smad3 and NF-κB (P6 5) m RN A in H O-8910 PM cells, and to explore its mechanism of anti-tumor invasion and metastasis. Methods: The expression of NF-κB (P6 5) and Smad3 protein was detected by Western blot. The expression of NF-κB (P6 5) m RNA was analyzed by RT-PCR. RESULTS: After treated with 2 ~ 10μmol / L cantharidin for 24 h, the expression of N F-κB (P6 5) and Smad3 protein and NF-κB (P6 5) m RN A (P <0.01), and showed a dose-response effect. Conclusion: The anti-tumor invasion and metastasis of cantharidin are related to the inhibition of the expression of NF-κB (P6 5), Smad3 protein and NF-κB (P6 5) m RNA.