【摘 要】
：
Reticulons (RTN) are endoplasmic reticulumassociated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neur
【机 构】
：
National Laboratory of Medical Molecular Biology,Department of Neurobiology,Peking Union Medicine Co
【基金项目】
：
国家自然科学基金;国家高技术研究发展计划(863计划);国家重点基础研究发展计划(973计划)
论文部分内容阅读
Reticulons (RTN) are endoplasmic reticulumassociated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. At least four different RTN genes have been identified in mammals, but in most cases,the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are still elusive. In the present study,mouse reticulon 3 (mRTN3) is cloned and its expression pattern in a variety of tissues is investigated. Three alternatively spliced transcripts of 1.8, 2.8 and 4.2 kb are revealed by Northern blotting hybridization. The 1.8 and 2.8 kb transcripts are expressed in many tissues. The 2.8 kb transcript has a high level in brain and the 4.2 kb transcript is only found in brain. In situ hybridization and immunohistochemical analysis indicated its high expression in non-glial cells in some particular region of mouse central nervous system, such as hippocampus, sub-thalamus nucleus, thalamus nucleus and cerebrum cortex.
其他文献
Human FⅨ expression vector pCMVⅨ was packaged by EffecteneTM reagent and injected into mice seminiferous tubules with glass pipettes. The expressional frame o
The p49 gene from baculovirus Spodoptera littoralis nucleopolyhedrovirus (SlNPV) was able to suppress apoptosis of Sf9 cells induced by virus infection. Ectopic
Spatio-temporal pattern analysis of EEG is an important tool in brain research. An EEG pattern analysis system based on a hierarchical multi-method approach is
The transcripts of beet necrotic yellow virus RNA5 were prepared from the cloned cDNA in a bacteriophage T7 in vitro run-off transcription system. The RNA5 tran
对175株普通小麦与6D/6V代换系杂种当代幼胚培养再生植株进行谷草转氨酶同工酶分析，其中来自杂交组合遗4095×RW15（6D／6V代换系）的2个植株（编号分别为98R149和98R159）GOT—V2编码的
Heat shock response is a self-defense mechanism for protection of cells and organisms from a wide range of harmful stressors. Recent studies revealed that it is
构建了含人血清白蛋白（hALB）基因的cDNA全序列、内含子1以及山羊β－酪蛋白基因启动子和5’端上游调控序列在内的乳腺表达载体pcDNA3.1－GCALBm，并通过显微注射将其导入经过体外受精
利用微卫星标记技术分析了8个肉牛杂交亲本群体的遗传结构和遗传变异，预测了杂种优势。在此基础上，对18个肉牛杂交组合的实际杂交效果利用个体动物模型进行了评估，并提出了筛选