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构建了含人血清白蛋白（hALB）基因的cDNA全序列、内含子1以及山羊β－酪蛋白基因启动子和5’端上游调控序列在内的乳腺表达载体pcDNA3.1－GCALBm，并通过显微注射将其导入经过体外受精（IVF）的奶牛受精卵中，体外培养到桑椹胚后期，取出少许细胞，应用巢式PCR对其进行目的基因整合及性别的预鉴定，将10枚整合胚移植于8头受体母牛，结果有3头妊娠，受孕率为37.5％（3／8），其中2头于妊娠中期流产，另有1头足月分娩产下1头有人血清白蛋白基因整合的雄性转基因试管牛，转基因实验的总有效率为12.5％（1／8）。
A full-length cDNA sequence containing human serum albumin (hALB) gene, intron 1, and the goat β-casein gene promoter and the 5 ’upstream regulatory sequence were constructed, and the expression vector pcDNA3.1-GCALBm The cells were introduced into IVF cows by microinjection, cultured in vitro to the morula stage, a few cells were removed, nested PCR was used to carry out gene integration and sex pre-identification, and 10 integrated embryos Transplantation of 8 recipient cows resulted in 3 pregnancies with a conception rate of 37.5% (3/8), of which 2 were aborted during the second trimester of pregnancy and one full-term delivered one human serum albumin gene The total combined efficiency of transgenic male and female transgenic cattle was 12.5% (1/8).