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背景:许旺细胞是周围神经修复过程的重要细胞,而研究发现人羊膜细胞分泌的多种细胞因子能够促进许旺细胞增殖。目的:观察不同浓度人羊膜匀浆上清液对鼠许旺细胞(RSC96)生长的影响。方法:使用含体积分数20%胎牛血清的高糖DMEM培养基原代培养RSC96细胞株,传代至第2代用于实验研究。根据人羊膜匀浆上清液在培养基中的不同体积分数(0,10,15,20,25%)分组。结果与结论:人羊膜匀浆上清液的总蛋白浓度为675 mg/L,表皮生长因子、碱性成纤维生长因子和血管内皮生长因子浓度分别为(470.625±2.546),(4.121±0.026)和(0.172±0.002)ng/L。在培养第1-7天,10%和15%人羊膜匀浆上清液组的增殖率大于20%和25%人羊膜匀浆上清液组(P<0.05);10%、15%人羊膜匀浆上清液组显示出促进细胞增殖的作用,而20%、25%人羊膜匀浆上清液组显示出抑制细胞增殖的作用;各实验组的细胞活力与对照组接近(P>0.05)。提示人羊膜匀浆上清液低浓度时(10%和15%)具有促进RSC96增殖作用,高浓度时(20%和25%)抑制RSC96增殖。
BACKGROUND: Schwann cells are important cells in the process of peripheral nerve repair. However, many cytokines secreted by human amniotic cells have been found to promote the proliferation of Schwann cells. Objective: To observe the effects of different concentrations of human amniotic homogenate supernatant on the growth of mouse Schwann cells (RSC96). Methods: The RSC96 cell line was cultured in high glucose DMEM containing 20% fetal bovine serum and passaged to passage 2 for experimental study. Human amnion homogenate supernatants were divided into different volume fractions (0, 10, 15, 20, 25%) in culture medium. RESULTS AND CONCLUSION: The total protein concentration of human amniotic homogenate supernatant was 675 mg / L, the concentrations of epidermal growth factor, basic fibroblast growth factor and vascular endothelial growth factor were (470.625 ± 2.546) and (4.121 ± 0.026) And (0.172 ± 0.002) ng / L respectively. The proliferation rates of 10% and 15% human amniotic homogenate supernatant group were higher than 20% and 25% human amniotic homogenate supernatant group (P <0.05) on day 1-7. 10% and 15% The amniotic homogenate supernatant group showed the effect of promoting cell proliferation, while the 20%, 25% human amniotic homogenate supernatant group showed the effect of inhibiting cell proliferation; the cell viability of each experimental group was similar to that of the control group (P> 0.05). The results showed that human amniotic homogenate supernatant at low concentrations (10% and 15%) promoted the proliferation of RSC96, and inhibited RSC96 proliferation at high concentration (20% and 25%).