论文部分内容阅读
目的:比较荧光偏振免疫分析法(FPIA)和高效液相色谱法(HPLC)监测丙戊酸血清药物浓度的相关性。方法:收集癫痫患者服丙戊酸后的稳态谷浓度血样,分别用FPIA法和HPLC法进行测定,考察二种测定方法的相关程度。结果:以HPLC法测定结果(x)与FPIA法测定结果(y)所作线性回归方程如下:y=1.8163+0.8407x(r=0.944),二种方法测定丙戊酸血清药物浓度结果差异有统计学意义(P<0.05)。结论:FPIA法和HPLC法测定丙戊酸血药浓度结果差异具有统计学意义,在丙戊酸治疗药物监测中应予以关注并作相应调整。
OBJECTIVE: To compare the correlation of serum valproic acid concentrations by fluorescent polarization immunoassay (FPIA) and high performance liquid chromatography (HPLC). Methods: Blood samples of steady-state trough valsartan were collected from patients with epilepsy. FPIA and HPLC were used to determine the correlation between the two methods. Results: The linear regression equation determined by HPLC method (x) and FPIA method (y) was as follows: y = 1.8163 + 0.8407x (r = 0.944). There were statistical differences between the two methods in the determination of serum valproic acid concentration Significance (P <0.05). Conclusion: The difference of plasma concentration of valproic acid between FPIA and HPLC was statistically significant, and attention should be paid to the monitoring of valproic acid drug therapy and adjusted accordingly.