FIA法测定脑清片中氨基比林含量

• 作 者: 高文兰 米慕贞 杨芳
• 机 构: 沈阳药学院基础部
• 出 处: 沈阳药学院学报
• 发表日期: 1994年04期
• 关键词: FIA法 脑清 流动注射分析 标示量 药典法 相对标准差 变异系数 系数倍率法 精密量取 采样频率

本文报道脑清片中氨基比林与硫酸铁（Ⅲ）铵形成有色络合物，在λ５００ｎｍ处，利用流动注射分析法测定其含量，标准曲线浓度的线性范围为２５０～６５μｇ／ｍＬ，其相关系数为０．９９９６，样品含量测定结果为标示量的９９．６％，相对标准差（ＲＳＤ）或变异系数（ＣＶ）为０．４１％（ｎ＝１５）．平均回收率为１００．０％，相对标准差或变异系数为０．４３％（ｎ＝１５）．与药典法相比下检验和ｔ检验均无显著性差异．采样频率为８０样／小时，该方法简便、快速、准确．

In this paper, we report the formation of a colored complex of aminopyrine and ammonium ferric (III) sulfate in Naoxing tablets by flow injection analysis at λ500 nm. The linear range of the standard curve concentration is 250-65 μg / mL. The coefficient was 0.9996. The content of the sample was 99.6% of the labeled amount, and the relative standard deviation (RSD) or coefficient of variation (CV) was 0.41% (n = 15). The average recovery was 100.0% with a relative standard deviation or coefficient of variation of 0.43% (n = 15). Compared with the Pharmacopoeia test and t test were no significant differences. Sampling frequency of 80 samples / hour, the method is simple, fast and accurate.