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AIM:To determine the methylation status and aberrant expression of some secreted frizzled-related protein(SFRP) genes in pancreatic cancer and explore their role in pancreatic carcinogenesis.METHODS:Methylation status and expression of SFRP genes were detected by methylation-specific PCR(MSPCR) and reverse-transcription PCR(RT-PCR) respectively.RESULTS:The frequencies of methylation for SFRP genes 1,2,4,5 were 70%,48.3%,60% and 76.7% in pancreatic cancer samples,and 21.7%,20%,10% and 36.7% in matched cancer adjacent normal tissue samples,respectively(χ2 = 28.23,P < 0.0001 for SFRP gene 1;χ2 = 10.71,P = 0.001 for SFRP gene 2;χ2 = 32.97,P < 0.0001 for SFRP gene 4;χ2 = 19.55,P < 0.0001 for SFRP gene 5).Expression loss of SFRP genes 1,2,4 and 5 was found in 65%,40%,55% and 71.7% of 60 pancreatic cancer samples,and 25%,15%,18.3% and 31.7% of matched cancer adjacent normal tissue samples,respectively(χ2 = 19.39,P < 0.0001 for SFRP gene 1;χ2 = 9.40,P = 0.002 for SFRP gene 2;χ2 = 17.37,P < 0.0001 for SFRP gene 4;χ2 = 19.22,P < 0.0001 for SFRP gene 5).SFRP gene 1 was methylated but not expressed in PC-3 and PANC-1,SFRP gene 2 was methylated but not expressed in PANC-1 and CFPAC-1,SFRP gene 4 was methylated but not expressed in PC-3,and SFRP gene 5was methylated but not expressed in CFPAC-1.
AIM: To determine the methylation status and aberrant expression of some secreted frizzled-related protein (SFRP) genes in pancreatic cancer and explore their role in pancreatic carcinogenesis. METHODS: Methylation status and expression of SFRP genes were detected by methylation-specific PCR (MSPCR ) and the frequencies of methylation for SFRP genes 1,2,4,5 were 70%, 48.3%, 60% and 76.7% in pancreatic cancer samples, and 21.7% P <0.0001 for SFRP gene 1; χ2 = 10.71, P = 0.001 for SFRP gene 2; χ2 = 32.97, P <0.0001 for SFRP gene 4; χ2 = 19.55, P <0.0001 for SFRP gene 5) .Expression loss of SFRP genes 1, 2, 4 and 5 was found in 65%, 40%, 55% and 71.7% of 60 pancreatic cancer samples, and (Χ2 = 19.39, P <0.0001 for SFRP gene 1; χ2 = 9.40, P = 0.002 for SFRP gene 2; χ2 = 17.37, P <0.0001 for SFRP gene 4; χ2 = 19.22, P <0.0001 for SFRP gene 5). SFRP gene 1 was methylated but not expressed in PC-3 and PANC-1, SFRP gene 2 was methylated but not expressed in PANC-1 and CFPAC-1, SFRP gene 4 was methylated but not expressed in PC-3, and SFRP gene 5was methylated but not expressed in CFPAC-1.