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在动物及人类红细胞体外及体内研究的一些观察中,提示维生素E可保护红细胞免于氧化剂诱发的损害,而继发于葡萄糖-6-磷酸脱氢酶(G-6-PD)缺陷的慢性溶血,归因于氧化剂所诱发的红细胞膜的损坏。作者等遂研究和观察维生素E对G-6-PD缺陷伴有慢性溶血患者的疗效。以往在这方面的一些研究所报道的结果多不一致。方法:对象为3例G-6-PD完全表现型突变伴高速慢性溶血的男性成人患者。口服消旋α-醋酸生育酚30IU/日/kg。治前及治疗4周后用高性能液体色层分析法测定血浆与细胞(血小板及红细胞)的α-生育酚水平。用肝素抗凝血分离红细胞膜行凝胶电泳,再用凝胶滤液层析法计测膜多肽的聚集(分子量>50×10~6道尔顿)。用de la Huerga法测定血浆总类脂质的
In some observations in vitro and in vivo of animal and human erythrocytes, it is suggested that vitamin E protects erythrocytes from oxidant-induced damage whereas chronic hemolysis secondary to defects in glucose-6-phosphate dehydrogenase (G-6-PD) , Due to oxidant-induced destruction of erythrocyte membranes. The authors then studied and observed vitamin E on G-6-PD defects associated with chronic hemolytic patients. In the past, some studies in this area reported more than the same results. Methods: The subjects were 3 adult male patients with G-6-PD complete phenotype with high-speed chronic hemolysis. Oral racemic α-tocopheryl acetate 30IU / day / kg. Plasma and cell (platelets and erythrocytes) α-tocopherol levels were determined by high performance liquid chromatography before and after 4 weeks of treatment. Heparin anticoagulant red blood cell membrane separation gel electrophoresis, and then measured by gel filtration chromatography membrane polypeptide aggregation (molecular weight> 50 × 10 ~ 6 Dalton). Determination of total plasma lipids by de la Huerga method