催乳素对培养基中的卵巢间质细胞黄体生成激素刺激雄性激素合成的抑制作用:作用机制的研究

• 作 者: 郁士浩
• 出 处: 国外医学(计划生育分册)
• 发表日期: 1984年02期
• 关键词: 卵巢间质细胞 催乳素 黄体生成激素 雄性激素 卵泡膜细胞 抑制作用 切除垂体 作用机制 培养基 卵巢

作者们曾报导关于催乳素(PRL)可抑制黄体生成素(LH)刺激卵巢间质细胞和卵泡膜细胞产生雄性激素的作用。本实验设计主要是为了进一步了解其作用机理。实验方法;将切除垂体的未成熟小白鼠的卵巢切成4～6块,然后加入无血清的特殊培养基中37°90分钟,将培养分散的细胞最后制备成每一培养皿中含有10~6个细胞,再继续培养8天。每2天更换培养基一次,更换时加入新鲜激素。以这种培养的细胞作下列各项实验。单加入 LH(100ng/ml)4天后雄酮合成比

The authors have reported on the role of prolactin (PRL) in inhibiting the production of androgens by luteinizing hormone (LH) stimulation of ovarian stromal cells and theca cells. The experimental design is mainly to understand its mechanism of action. Experimental methods; ovariectomized hypophysectomized ovary cut into 4 to 6, and then added to serum-free special medium 37 ° 90 minutes, the cultured cells were finally prepared into petri dishes each containing 10 ~ 6 cells, then continue to cultivate for 8 days. Replace the medium once every 2 days and add fresh hormone when changing. The cultured cells were used for the following experiments. After 4 days of LH alone (100 ng / ml), the ratio of androsterone synthesis was increased