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目的:研究机械拉伸刺激对大鼠骨髓间充质干细胞迁移行为的影响并探讨其相关分子机制。方法:应用单轴机械拉伸加载装置考察不同条件的周期拉伸刺激对大鼠骨髓间充质干细胞迁移行为的影响,采用Transwell和划痕法评价细胞迁移能力,采用明胶酶谱法检测基质金属蛋白酶-2,-9(MMP-2,-9)表达的变化。结果:适宜的拉伸刺激可以明显促进大鼠骨髓间充质干细胞的迁移能力,1 Hz、10%应变拉伸8 h后可以使细胞迁移数量增加到对照组的1.58倍。拉伸刺激诱导骨髓间充质干细胞基质金属蛋白酶-2,-9(MMP-2,-9)表达。抑制剂GM6001抑制了拉伸诱导的MMP-2,-9分泌增加,同时抑制了拉伸刺激对细胞迁移的促进作用。结论:机械拉伸刺激影响大鼠骨髓间充质干细胞的迁移行为,MMP-2,-9在此过程中可能起着重要介导作用。
Objective: To study the effect of mechanical stretch stimulation on the migration of rat bone marrow mesenchymal stem cells and to explore the molecular mechanisms involved. Methods: The uniaxial mechanical tensile loading device was used to investigate the effect of cyclic stretching stimulation on the migration of rat bone marrow mesenchymal stem cells under different conditions. Transwell and scratching assay were used to evaluate the migration ability of cells. Gelatin zymography Protease-2, -9 (MMP-2, -9) expression changes. Results: Tensile stimulus could significantly promote the migration of rat bone marrow-derived mesenchymal stem cells. After being stretched at 10% strain at 1 Hz for 1 h, the number of migrated cells increased to 1.58 times that of the control group. Tensile stimulation induces the expression of matrix metalloproteinase-2, -9 (MMP-2, -9) in bone marrow mesenchymal stem cells. Inhibitor GM6001 inhibited the elongation-induced increase of MMP-2 and -9 secretion, and inhibited the stimulation effect of stretch stimulation on cell migration. Conclusion: Tensile mechanical stimulation can affect the migration of rat bone marrow mesenchymal stem cells. MMP-2 and -9 may play an important mediating role in this process.