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1978～79年,我们在甘肃省的陇南、河西和甘南等地两次考察植物病毒病害,行程几千公里,经历了不同作物生态区。观察到在外观上表现植物病毒病害和植物黄化病害共有53种。包括粮食作物、果树林木、豆类、蔬菜、瓜果、其他作物和草类。并对八种样品作电子显微镜观察,从病株中发现病毒质粒。 1.玉米矮花叶病:将甘谷地区玉米矮花叶病株样品,低温冰冻,捣碎,柠檬酸缓冲液抽提,聚乙二醇沉淀,差速离心,制备成初步净化样品,负染、电镜观察。可见大量的
From 1978 to 1979, we investigated plant virus diseases twice in Longnan, Hexi and Gannan counties in Gansu Province. We traveled thousands of kilometers and experienced different crop ecological zones. A total of 53 species of plant diseases and plant diseases were observed in appearance. Includes food crops, fruit trees, beans, vegetables, fruits, other crops and grasses. Eight kinds of samples were observed under electron microscope, and virus plasmids were found from diseased plants. 1. Maize dwarf mosaic disease: The sample of maize dwarf mosaic virus in Gangu region was frozen and crushed, extracted with citric acid buffer, precipitated with polyethylene glycol and differentially centrifuged to prepare a preliminary purified sample with negative staining Electron microscopy. Visible a lot