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目的:观察自拟处方CQM对光化学诱导的三叉神经痛模型大鼠镇痛效应及其对脑内兴奋性递质谷氨酸(Glu)的影响。方法:将雄性SD大鼠随机分为假手术对照组和光化学诱导三叉神经痛手术组,后者再分为模型组、加巴喷丁组(100mg·kg-1),CQM低、高剂量组(35,70 mg·kg-1)。采用机械痛敏测试的方法评价大鼠的疼痛行为,以机械痛阈反映药效。脑内微透析方法采集大鼠纹状体细胞外液,高效液相-荧光法检测其中Glu水平。结果:与对照组相比,光化学诱导的三叉神经痛模型大鼠的机械痛敏感行为明显增强(P<0.01),脑内Glu浓度显著升高(P<0.05)。与模型组相比,各用药组机械痛阈均有上升,其中CQM高剂量组在3个时间点显著升高(P<0.05),最高达到(23±7.3)g,加巴喷丁组在2个时间点显著升高(P<0.05),最高达到(20.5±9.2)g。而反映大鼠脑内Glu浓度的指标各用药组均显著低于模型组(P<0.05)。结论:CQM能减轻光化学诱导的三叉神经痛模型大鼠的机械疼痛敏感行为,其镇痛效应可能与降低纹状体细胞外液中Glu水平有关。
Objective: To observe the analgesic effect of self-prescribed CQM on photochemically induced trigeminal neuralgia in rats and its effect on glutamate (Glu), an excitatory neurotransmitter in the brain. Methods: Male Sprague-Dawley rats were randomly divided into sham operation group and photochemically induced trigeminal neuralgia group. The latter group was further divided into model group, gabapentin group (100 mg · kg -1), CQM low dose group (35 mg · kg -1) 70 mg · kg -1). The mechanical pain sensitivity test was used to evaluate the pain behavior in rats, and the mechanical pain threshold was used to reflect the efficacy. The intracerebral microdialysis method was used to collect the extracellular fluid of rat striatum, and the Glu level was detected by HPLC-fluorescence method. Results: Compared with the control group, the mechanical pain sensitivity in photochemically induced trigeminal neuralgia model rats was significantly enhanced (P <0.01), and the concentration of Glu in brain increased significantly (P <0.05). Compared with the model group, the mechanical pain threshold of each drug group increased, of which CQM high dose group was significantly increased at 3 time points (P <0.05), the highest (23 ± 7.3) g, gabapentin group at 2 time Point was significantly higher (P <0.05), up to (20.5 ± 9.2) g. The indicators that reflect the concentration of Glu in rat brain were significantly lower than those in model group (P <0.05). CONCLUSION: CQM can reduce the mechanical pain-sensitive behavior of photochemically induced trigeminal neuralgia model rats, and its analgesic effect may be related to the decrease of Glu level in extracellular fluid of striatum.