Cloning of human 15ku selenoprotein gene from H9 T cells

• 作 者: Ke-JunNan Chun-LiLi Yong-Chang
• 机 构: DepartmentofMedicalOntology.FirstHospitalofXi',anJiaot0ngUniversity,TheUniversityofGeorgia,Depart
• 出 处: 世界胃肠病学杂志：英文版（电子版）
• 发表日期: 2003年8期
• 关键词: 硒蛋白基因 T细胞 聚合酶链反应 CDNA文库
• 基金项目: 陕西省科技研究发展计划

AIM: To clone human 15ku selenoprotein gene.METHODS: H9 human T cells were cultured in RPMI1640medium supplemented with 100 mL/L fetal calf serum. mRNA was isolated from the cells. cDNA library was constructed by RT-PCR. The human 15ku selenoprotein gene