论文部分内容阅读
目的 探讨动脉内膜损伤后内膜增殖灶中平滑肌细胞凋亡现象及其相关调控基因的表达。方洁 取血管内膜损伤后４小时、２４小时、７天、１４天、２８天、３个月、４个月时的兔动脉进行光镜、电镜检查、原位细胞凋亡标记实验(ＴＵＮＥＬ)以及ｃ－ｆｏｓ、ｃ－ｍｙｃ、ｐ５３ｍＲＮＡ原位杂交实验。结果电镜观察到凋亡细胞早期的表现;７~１２０天内膜中细胞凋亡的发生率仅为１%~ ２%左右;原位杂交显示７天组ｃ－ｆｏｓ、ｃ－ｍｙｃ呈阳性表达,以后表达强度逐渐下降直至转阴,与内膜细胞增殖动力学变化的趋势一致,ｐ５３仅在７天组有弱阳性表达。结论 动脉内膜损伤后增殖灶中细胞凋亡的作用非常微弱,在内膜增殖的形成中不是主要的影响因素。ｃ－ｆｏｓ、ｃ－ｍｙｃ、ｐ５３基因的表达与内膜中平滑肌细胞增殖动力学和细胞凋亡的变化趋势相吻合。
Objective To investigate the apoptosis of smooth muscle cells and the expression of related regulatory genes in the intimal hyperplasia after arterial intimal injury. Fang Jie The arteries of rabbits at 4, 24, 7, 14, 28, 3 and 4 months after injury were examined by light microscopy, electron microscopy and in situ cell apoptosis labeling (TUNEL) ) And c-fos, c-myc, p53 mRNA in situ hybridization experiments. Results The early apoptotic cells were observed by electron microscopy. The rate of apoptosis in the membrane was only about 1% -2% within 7-120 days. The in situ hybridization showed that the expression of c-fos and c-myc were positive on the 7th day , The intensity of expression gradually decreased until negative, consistent with the trend of proliferation dynamics of endometrial cells, p53 only weakly positive expression in the 7-day group. Conclusions Apoptosis in proliferative focus after arterial intimal injury is very weak, which is not the main factor in the formation of intimal hyperplasia. The expression of c-fos, c-myc and p53 genes coincided with the trend of intimal smooth muscle cell proliferation and apoptosis.