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目的:研究复方中药VI-28胶囊(即卫康胶囊,主要成分有人参、鹿茸、菟丝子)辅助环磷酰胺体内抑制肿瘤生长的作用及其机制。方法:C57BL/6纯系小鼠背部皮下接种Lewis肺癌细胞(LCC,每只106个细胞),同时按40mg/kg隔天腹腔注射环磷酰胺治疗。实验组灌胃VI-28(2%悬液,每只0.5ml,隔天给药)。第28天时取小鼠胸腺和肿瘤组织块,计算肿瘤指数和胸腺指数。取脾细胞测定细胞免疫功能。组织切片观察所形成实体瘤的病理学特征。结果:小鼠接种LLC细胞14d后在接种部位开始形成实体瘤,瘤块直径增加为1—1.8cm。与单纯化疗荷瘤组小鼠相比,VI-28辅助治疗组的胸腺较大,脾细胞对ConA反应性较强,形成实体瘤的瘤块较小且分化程度较高。结论:VI-28胶囊可有效地促进化疗小鼠免疫功能的恢复,帮助化疗药物抑制肿瘤生长,是一种有效的化疗辅助药。
Objective: To study the effect and mechanism of adjuvant cyclophosphamide on tumor growth in compound Chinese herbal medicine VI-28 capsules (ie Weikang capsule, main components of which are ginseng, velvet antler and dodder). METHODS: C57BL / 6 mice were inoculated subcutaneously with Lewis lung carcinoma cells (LCC, 106 cells each) on the back of the mice and treated intraperitoneally with 40mg / kg cyclophosphamide at the same time. The experimental group was orally administered with VI-28 (2% suspension, each 0.5 ml, administered on alternate days). On the 28th day, the thymus and tumor tissue of mice were taken and the tumor index and thymus index were calculated. Spleen cells were taken to measure cellular immune function. Tissue sections were observed pathological features of solid tumors formed. Results: After 14 days of inoculation of LLC cells, solid tumors started to form in the inoculation site. The diameter of the tumor mass increased from 1 to 1.8 cm. The thymus of VI-28 adjuvant therapy group was larger than that of chemotherapy-alone tumor-bearing mice, and the spleen cells were more reactive to ConA. The tumor mass with solid tumor formed was smaller and the degree of differentiation was higher. Conclusion: VI-28 capsule can effectively promote the recovery of immune function in chemotherapy mice and help chemotherapy drugs inhibit tumor growth. It is an effective adjuvant chemotherapy.