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采用室内培养实验,观测淹水厌氧条件下分别添加及共同添加葡萄糖和不同制备来源的腐殖酸,对红壤中铁的异化还原作用的影响.结果表明,红壤单独培养条件下,Fe(Ⅱ)浓度培养前后没有发生变化.添加葡萄糖促进了铁的异化还原,培养至12 d其Fe(Ⅱ)浓度为培养前的25倍.腐殖酸不能作为电子供体促进铁的异化还原,单独添加时红壤中Fe(Ⅱ)浓度没有发生变化,而同时添加葡萄糖情况下,培养前期促进而后期减弱铁的异化还原,其Fe(Ⅱ)浓度增幅仅为单独添加葡萄糖处理的35%.腐殖酸的浓度对红壤中铁的异化还原作用有影响,浓度为2.00 g/kg时培养前期促进而后期减弱铁的异化还原,低浓度时(0.20和0.02 g/kg)影响很小.不同制备来源的腐殖酸对红壤中铁异化还原过程的影响不同.培养前期,从山西大同风化煤(HAs)、河南巩县褐煤(HAh)和云南昆明滇池底泥(HAk)中提取的腐殖酸都促进了红壤中铁的异化还原;培养后期,HAk依然发挥促进作用,其Fe(Ⅱ)浓度始终高于G处理,而添加HAs和HAh的处理培养至7 d Fe(Ⅱ)仅为单独添加葡萄糖处理的14%和25%,减弱了铁的异化还原.
The effects of adding humic acid with glucose and different preparation sources on the dissimilatory reduction of iron in red soils were investigated by indoor culture experiments under anaerobic conditions of flooding.The results showed that Fe (Ⅱ) The concentration of Fe did not change before and after the culture, and the addition of glucose promoted the dissimilatory reduction of iron, and the concentration of Fe (Ⅱ) was 25 times higher than that before culturing at 12 days.Humanic acid could not promote the dissimilatory reduction of iron as an electron donor, In the red soil, the concentration of Fe (Ⅱ) did not change, but at the same time, the concentration of Fe (Ⅱ) increased only 35% of the glucose alone when the glucose was added at the same time. The effect of concentration on the dissimilatory reduction of iron in red soil was promoted at the initial concentration of 2.00 g / kg, but decreased at the low concentration (0.20 and 0.02 g / kg) The effect of acid on the dissimilatory reduction of iron in red soil was different.Under the cultivation stage, the humic acids extracted from HAs in Shanxi Datong, HAh in Henan and HAk in Kunming Dianchi Lake in Yunnan Province all promoted In the late stage of cultivation, HAk still played a promoting role, and the concentration of Fe (Ⅱ) was always higher than that of G treatment. However, the treatment with HA and HAh for 7 days was only supplemented with glucose alone 14 % And 25%, weakened the dissimilatory reduction of iron.