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目的建立板蓝根颗粒及药材中腺苷和RS-告伊春的测定方法。方法RS-告依春的测定采用Dikma Diamonsil C18色谱柱(200mm×4.6mm,5μm);流动相:乙腈-水-三乙胺(10∶90∶0.2,用冰醋酸调pH值5.2);柱温:室温;体积流量:0.8mL/min;检测波长:245nm;腺苷的测定采用Dikma Diamonsil C18色谱柱(200mm×4.6mm,5μm);流动相:乙腈-水(5∶95);柱温:室温;体积流量:1.0mL/min;检测波长:260nm。结果RS-告依春的线性范围为0.676~13.1μg/mL;板蓝根颗粒和药材的平均回收率分别为101.66%、99.74%,RSD分别为0.9%、1.4%(n=6);腺苷的线性范围分别0.005~0.05mg/mL,板蓝根颗粒和药材的平均回收率分别为97.76%、97.32%,RSD分别为1.62%、0.62%(n=6)。结论所建立的方法准确、可靠,可用于板蓝根颗粒及药材的质量控制。
Objective To establish a method for the determination of adenosine and RS-Yichun in Banlangen granules and herbs. METHODS RS-Caiyichun was determined using a Dikma Diamonsil C18 column (200mm×4.6mm, 5μm); mobile phase: acetonitrile-water-triethylamine (10:90∶0.2, adjusted to pH 5.2 with glacial acetic acid); column Temperature: room temperature; volumetric flow rate: 0.8 mL/min; detection wavelength: 245 nm; measurement of adenosine using Dikma Diamonsil C18 column (200 mm×4.6 mm, 5 μm); mobile phase: acetonitrile-water (5:95); column temperature : room temperature; volumetric flow rate: 1.0 mL/min; detection wavelength: 260 nm. Results The linear range of RS-Yiyichun was 0.676~13.1μg/mL. The average recoveries of Radix Isatidis and Radix Isatidis were respectively 101.66% and 99.74%. The RSDs were 0.9% and 1.4%, respectively (n=6); adenosine The linear ranges of 0.005-0.05 mg/mL, the average recoveries of Radix isatidis and medicinal materials were 97.76% and 97.32%, respectively, and the RSDs were 1.62% and 0.62% (n=6), respectively. Conclusion The established method is accurate and reliable and can be used for the quality control of Radix Isatidis granules and medicinal materials.