大鼠消化道肌间神经丛中一氧化氮合酶的分布

• 作 者: 彭曦 冯晋斌 汪仕良
• 机 构: 第三军医大学附属西南医院烧伤研究所,解放军２６４医院烧伤科
• 出 处: 华人消化杂志
• 发表日期: 1998年03期
• 关键词: 大鼠 消化道 肌间神经丛 一氧化氮合酶

目的了解大鼠消化道肌间神经丛中一氧化氮合酶（ＮＯＳ）阳性神经元的分布规律．方法采用ＮＡＤＰＨ黄递酶（ＮＤＰ）组化技术在整装铺片上观察了大鼠消化道肌间神经丛中ＮＯＳ阳性神经元的形态特征并对其分布进行定量研究．结果整个消化道肌间神经丛中均有ＮＯＳ阳性神经元分布，密度（个／ｍｍ２）分别为１８±２５（食管），３１４±４７（胃），２６４±３１（十二指肠），２９５±３９（空肠）。３３７±４４（回肠），６７±２３（盲肠），３８６±４１（升结肠），４８７±５３（降结肠）和３６２±３３（直肠）．神经纤维分布仍以降结肠最密集（＋＋＋＋），食管最稀疏（＋）．ＮＯＳ神经元形态基本类似，大小差异较大，绝大部分神经元位于神经节内，每个神经节中ＮＯＳ神经元２～８个．多数神经元之间有突起相互连接，形成网络．结论消化道中ＮＯＳ神经元的分布与该处的生理功能相吻合，并可能与许多胃肠疾病的发生及发展密切相关

Objective To investigate the distribution of nitric oxide synthase (NOS) positive neurons in the gastrointestinal myenteric plexus of rats. Methods The morphological characteristics of NOS positive neurons in the gastrointestinal myenteric plexus of rats were observed by NADPH-diaphorase (NDP) staining technique and the distribution of them was quantitatively studied. Results NOS positive neurons were found in all myenteric plexus of the digestive tract, with a density of 18 ± 25 (esophagus), 314 ± 47 (stomach), 264 ± 3  1 (duodenum), 29  5 ± 3  9 (jejunum). 33.7 ± 4.4 (ileum), 67 ± 23 (cecum), 386 ± 41 (ascending colon), 487 ± 53 (descending colon) and 362 ± 3  3 (rectum). Nerve fiber distribution is still the most intensive descending colon (++++), the most sparse esophagus (+). NOS neurons are basically similar in shape and size, with the vast majority of neurons located in the ganglia, 2 to 8 NOS neurons in each ganglion. Most neurons have protrusions connected to each other to form a network. Conclusions The distribution of NOS neurons in the digestive tract is consistent with the physiological function of the gastrointestinal tract and may be closely related to the occurrence and development of many gastrointestinal diseases