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目的建立烫伤合剂中盐酸小檗碱的含量测定方法。方法色谱柱为Inertsil C8-3柱(4.6 mm×250 mm,5μm),流动相为0.05 mol.L-1磷酸二氢钠溶液-乙腈-磷酸(70∶30∶0.08);流速为1 mL.min-1,柱温:35℃,检测波长为260 nm。结果盐酸小檗碱在5.28~52.8 mg.L-1内峰面积与浓度呈良好的线性关系,Y=2.45×104X+4.91×103(r=0.999 4),平均回收率为100.30%,RSD为1.24%(n=6)。结论方法简便、准确、重复性好,可作为控制产品质量的定量依据。
Objective To establish a method for the determination of berberine hydrochloride in scald mixture. Methods The column was Inertsil C8-3 (4.6 mm × 250 mm, 5 μm). The mobile phase was 0.05 mol·L-1 sodium dihydrogen phosphate solution-acetonitrile-phosphoric acid (70:30:0.08). The flow rate was 1 mL. min-1, column temperature: 35 ℃, detection wavelength of 260 nm. Results The peak area of berberine hydrochloride in the range of 5.28 ~ 52.8 mg.L-1 showed a good linear relationship with the concentration of Y = 2.45 × 104X + 4.91 × 103 (r = 0.999 4). The average recovery was 100.30% 1.24% (n = 6). Conclusion The method is simple, accurate, reproducible and can be used as a quantitative basis to control product quality.