论文部分内容阅读
目的建立一种简便的RP-HPLC法测定全血中环孢素A(CsA)的浓度,为临床合理应用CsA提供依据。方法全血样品1 mL经乙醚提取,正己烷洗涤去干扰后,采用Zorbax SB-C18柱(4.6 mm×150 mm,3.5μm)分离,流动相为乙腈-甲醇-水-异丙醇(60∶10∶29∶1),流速1.5 mL.min-1,柱温70℃,检测波长210 nm;环孢素D(CsD)为内标。结果CsA全血浓度在25~1 600 ng.mL-1内线性关系良好(r=0.999 8),平均方法回收率99.51%,日内、日间RSD均小于5%,最低检测限5 ng.mL-1。结论本法简便快速,灵敏度高,重复性好,线性范围广。用于患者全血中CsA浓度监测,效果良好。
Objective To establish a simple and convenient RP-HPLC method for the determination of CsA in whole blood and provide a basis for clinical application of CsA. Methods Whole blood samples were extracted with diethyl ether and washed with n-hexane to separate the whole blood samples. The separated samples were separated on a Zorbax SB-C18 column (4.6 mm × 150 mm, 3.5 μm) using a mobile phase of acetonitrile-methanol-water- 10:29:1). The flow rate was 1.5 mL · min-1. The column temperature was 70 ℃ and the detection wavelength was 210 nm. CsD was used as internal standard. Results The linear range of CsA whole blood was 25 ~ 1 600 ng.mL-1 (r = 0.999 8). The average recovery rate of CsA was 99.51%. The intra-day and inter-day RSD were less than 5%, and the lowest detection limit was 5 ng.mL -1. Conclusion This method is simple and fast, high sensitivity, good repeatability and wide linear range. CsA concentration in patients with whole blood monitoring, the effect is good.