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目的 试图揭示血管内皮生长因子 (VEGF)和E2 6转录因子 (E2 6transformation specific 1,ETS 1)在乳腺癌组织中的表达规律 ,探讨其在血管生成和肿瘤浸润转移中的作用机制。方法 应用原位杂交和免疫组织化学链霉素抗生物素 过氧化物酶复合物法 (SP)法 ,检测 4 8例乳腺癌组织中VEGF和ETS 1的mRNA和蛋白的表达。结果 乳腺癌细胞高表达VEGFmRNA和蛋白 ,阳性率分别为 75 % (36 / 4 8)、70 8% (34/ 4 8) ,而血管内皮细胞几乎不表达 ;ETS 1既表达在乳腺癌细胞 ,也表达在血管内皮细胞。癌细胞中mRNA和蛋白表达阳性率分别为 85 4 % (41/ 4 8)、79 2 % (38/ 4 8) ;VEGF和ETS 1高表达组的血管密度明显高于低表达组 (均P <0 0 1) ;VEGF和ETS 1的表达与组织学分级和淋巴结转移密切相关 ,并且高表达组的微血管密度明显高于低表达组 (P <0 0 1)。结论 VEGF和ETS 1可促进乳腺癌血管形成 ,同时也促进肿瘤的浸润和转移 ;检测VEGF和ETS 1的表达可做为判定乳腺癌恶性度、浸润转移等生物学行为的参考指标
OBJECTIVE: To investigate the expression of vascular endothelial growth factor (VEGF) and E2 6 transcriptional factor 1 (ETS 1) in breast cancer and to explore its mechanism of action in angiogenesis and tumor invasion and metastasis. Methods In situ hybridization and immunohistochemical streptavidin-biotin peroxidase complex method (SP method) were used to detect the expression of VEGF and ETS 1 mRNA and protein in 48 cases of breast cancer. Results The positive rates of VEGF mRNA and protein in breast cancer cells were 75% (36/48) and 70 8% (34/48), respectively, while the expression of VEGF was almost undetectable. ETS 1 expressed both in breast cancer cells, Also expressed in vascular endothelial cells. The positive rates of mRNA and protein expression in cancer cells were 85 4% (41/48) and 79 2% (38/48), respectively. The vascular densities of VEGF and ETS 1 overexpression groups were significantly higher than those of low expression groups <0 01). The expression of VEGF and ETS 1 was closely related to histological grade and lymph node metastasis, and the microvessel density in high expression group was significantly higher than that in low expression group (P <0.01). Conclusions VEGF and ETS 1 can promote angiogenesis of breast cancer and promote the infiltration and metastasis of breast cancer. Detecting the expression of VEGF and ETS 1 can be used as a reference index to judge the biological behavior of breast cancer such as malignancy, invasion and metastasis