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目的了解深圳市HIV-1主要流行亚型Env基因V3环氨基酸序列变异特征。方法采用巢式聚合酶链反应(Nested-PCR)扩增HIV-1Env区,对扩增产物进行基因测序,应用MEGA软件对序列进行整理和分析。结果深圳市HIV-1主要基因亚型为01-AE亚型179例(54.41%),07-BC亚型86例(26.14%)。共发现8种V3环顶端四肽类型,其中主要类型为GPGQ和GPGR;07-BC、08-BC和C亚型顶端四肽全部为GPGQ,01-AE和B亚型顶端四肽具有多种形态且两亚型间的分布有统计学差异;GPGQ和GPGR在01-AE和C亚型的分布情况与全国相符,在B亚型中分布与全国相比有统计学差异;01-AE、B亚型V3区基因离散率明显高于其他亚型;B亚型V3区11和25位单独和同时被带正电荷氨基酸替代达19例和3例,而01_AE亚型有7例11位单独被正电荷取代,07-BC、08-BC、C亚型仅有1例11位单独被正电荷氨基酸取代。结论 2009年深圳市HIV-1毒株主要呈巨噬细胞嗜性,只有极少部分毒株转变为T细胞嗜性;B亚型相对其他亚型V3区具更大变异性。
Objective To understand the variation of the amino acid sequence of V3 loop of HIV-1 major HIV-1 Env gene in Shenzhen. Methods The HIV-1 Env region was amplified by nested-polymerase chain reaction (Nested-PCR), and the amplified products were sequenced. The sequence was analyzed by MEGA software. Results The major subtypes of HIV-1 in Shenzhen were 179 (54.41%) in 01-AE subtype and 86 (26.14%) in 07-BC subtype. A total of eight V3 ring top tetrapeptides were found, of which the major types were GPGQ and GPGR; the top tetrapeptides for 07-BC, 08-BC and C subtypes were all GPGQ, and the top tetrapeptides for 01-AE and B subtypes had multiple Morphology and the distribution between the two subtypes were statistically different; GPGQ and GPGR distribution in the 01-AE and C subtypes consistent with the country, the distribution in the B subtype has a statistically significant difference compared with the national; 01-AE, The genotype V3 gene divergence in subgroup B was significantly higher than those in other subtypes. In subgroup B and subgroups, 11 and 25 were independently and simultaneously replaced by positively charged amino acids in 19 and 3 cases, respectively. Substituting positive charges, only one of the 11 in 07-BC, 08-BC, and C subtypes was replaced by a positively charged amino acid alone. Conclusion In 2009, HIV-1 strains in Shenzhen were predominantly macrophage-like and only a few of them were converted to T cell tropism. Type B was more variable than other subtypes of V3.