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目的:评估不同免疫状态下和抗病毒治疗后HBV慢性感染者外周血液中T细胞亚群比例情况及细胞因子水平。方法:选择进入本院接受诊治的54例HBV慢性感染患者,检测其不同免疫状态下和抗病毒治疗后外周血细胞的T细胞亚群比例情况及细胞因子水平,并选择20例同期健康人员的外周血样进行检测,比对各项指标数据。结果:非活动型HBV携带组、免疫耐受组、免疫活化组感染病例CD8~+T细胞ALT的实际含量高出健康人员,CD4~+T细胞实际含量少于对照组(P<0.05)。免疫活化组TNF-α、IL-6、IFN-γ、TL-10的实际浓度高出免疫耐受组、参照组,差异较大(P<0.05)。结论:HBV慢性感染者处在不同免疫状态时,检测其外周血液中T细胞亚群比例情况及细胞因子水平,能为重建免疫性功能机制提供关键的检测指标。
OBJECTIVE: To assess the percentage of T-cell subsets and cytokines in peripheral blood of patients with chronic HBV infection under different immunological conditions and after antiviral therapy. Methods: Totally 54 patients with chronic HBV infection admitted to our hospital were enrolled in this study. The proportion of T lymphocyte subsets and cytokines in peripheral blood cells of different immune states and antiviral therapy were measured. The peripheral Blood samples were tested, compared to the index data. Results: The actual content of ALT in non-active HBV carriers, immunocompromised patients and immunocompromised patients was higher than that in healthy volunteers. The actual content of CD4 ~ + T cells in CD8 ~ + T cells was lower than that in control group (P <0.05). The actual concentrations of TNF-α, IL-6, IFN-γ and TL-10 in immune activated group were significantly higher than those in immune tolerance group and reference group (P <0.05). CONCLUSIONS: In patients with chronic hepatitis B virus infection, the detection of T lymphocyte subsets and cytokines in peripheral blood may provide key diagnostic indicators for the reconstruction of immune functional mechanisms.