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RNA干扰是一项新近发现的通过向细胞内引入同源双链siRNA,在mRNA水平上特异性使基因沉默的技术。有丝分裂激酶(PLK-1)是哺乳动物细胞有丝分裂过程中最重要的调节因子之一,其表达量与多种人类肿瘤发生发展密切相关。PLK-1的丢失可使 M期细胞增多,两极纺锤体形成阻滞,细胞周期蛋白 B1聚积。研究者利用阳离子脂质体将PLK-1siRNA 导入膀胱癌细胞,观察PLK-1表达量的变化及其在体内外对膀胱癌的抑制作用,从而探讨膀胱内给予 PLK-1 siRNA-脂质体复合物对膀胱癌的治疗价值。
RNA interference is a newly discovered technique that specifically silences a gene at the mRNA level by introducing homoduplexed siRNA into a cell. Mitochondrial kinase (PLK-1) is one of the most important regulatory factors in mammalian cell mitosis, and its expression is closely related to the development of many human tumors. Lack of PLK-1 can increase the number of M-phase cells, bipolar spindle block formation, accumulation of cyclin B1. The researchers used cationic liposomes to introduce PLK-1 siRNA into bladder cancer cells, observed the change of PLK-1 expression and its inhibitory effect on bladder cancer in vitro and in vivo, so as to explore the effect of intra-bladder administration of PLK-1 siRNA-liposome complex Therapeutic value of bladder cancer.