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目的研究伽玛刀照射对癫痫大鼠的放射生物学作用,探讨伽玛刀治疗癫痫的作用机制。方法利用海人酸制备癫痫大鼠模型50只(癫痫组25只,伽玛刀照射组25只),对照组为正常大鼠25只(注射生理盐水),观察各组行为学改变;采集伽玛刀照射后1d、1w、2w、4w、8w后各组大鼠海马细胞外液,并测定Glu和GABA含量及观察海马超微结构变化。结果 1w后,照射组和癫痫组GABA含量均低于对照组;2w后,照射组和癫痫组Glu含量均高于对照组;4w后,照射组Glu含量低于癫痫组,GABA含量高于癫痫组;差异均具有统计学意义(P<0.05)。海马组织超微结构显示照射组早中期与癫痫组基本一致,其晚期部分结构恢复,线粒体修复较为明显。结论伽玛刀照射可调节海马中Glu与GABA以达到新的平衡,并在癫痫发作引起的病理性损伤修复中起到重要作用。
Objective To study the radiobiological effects of gamma knife irradiation on epileptic rats and to explore the mechanism of gamma knife treatment of epilepsy. Methods Fifty epileptic rats (25 epilepsy group and 25 gamma knife group) were prepared by using kainate acid. The control group consisted of 25 normal rats (injected with normal saline). The behavioral changes of each group were observed. The hippocampal extracellular fluid of rats in each group were exposed to 1, 2, 4, 8 weeks after the scalpel irradiation, and the contents of Glu and GABA were determined and the changes of ultrastructure in the hippocampus were observed. Results After 1 w, the GABA levels in the irradiated group and the epilepsy group were lower than those in the control group. After 2 weeks, the content of Glu in the irradiated group and the epileptic group was higher than that in the control group. After 4 weeks, the content of Glu in the irradiated group was lower than that in the epilepsy group, Group; the differences were statistically significant (P <0.05). The ultrastructure of hippocampus showed that the early and middle stages of irradiation group were basically the same as those of epilepsy group, and the ultrastructure of the hippocampus was recovered and the mitochondrial repair was obvious. Conclusion Gamma knife irradiation can regulate Glu and GABA in the hippocampus to reach a new equilibrium and play an important role in the repair of pathological damage caused by seizures.