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棕色固氮菌(Azotobacser Vinelandii—230)中与固氮酶共存的氢酶属于单向吸氢的氢酶,其中以粒子态存在者约占其总活力的78％,可溶态占22％。此二态酶对冷热稳定,最适反应温度和利用多种电子受体吸氢等方面均相似,但在耐热性、温度曲线的形式及对某些电子受体的亲和性方面有些差异。乙炔、Na~+离子及氧对酶的吸氢活力有抑制作用。菌体的氢酶活力,有氨培养比无氨培养的低86％左右;大量氨培养的UW—45菌体仍有氢酶活性,但比氨耗尽的UW45菌体低55％左右。钨代替钼培养的菌体无固氮酶活性,其粗提液氢酶活性也极低。由此可见,氢酶活性与固氮酶活性有极为密切的关系。
Hydrogenase coexisting with nitrogenase in Azotobacter vinelandii-230 belongs to hydrogenase which is one-way hydrogen-absorbing, in which the particle form exists about 78% of its total activity and the soluble form accounts for 22%. The di-enzyme is stable to cold and heat, the optimum reaction temperature and hydrogen absorption using a variety of electron receptors and other aspects are similar, but in the heat resistance, the temperature curve in the form and some of the affinity of some electron acceptors difference. Acetylene, Na ~ + ions and oxygen on the hydrogen-absorbing activity of the enzyme inhibition. The bacterial activity of hydrogenase was about 86% lower than that of ammonia-free culture. UW-45 cells cultured in a large amount of ammonia still had the activity of hydrogenase, but about 55% lower than that of UW45 cells depleted in ammonia. Tungsten instead of molybdenum culture bacteria without nitrogenase activity, the crude extract hydrogenase activity is also very low. Thus, hydrogenase activity and nitrogenase activity has a very close relationship.