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采用重组DNA技术成功构建了Rb基因表达质粒。Rb基因片段来源于Rb cDNA克隆P2R4.7的BglⅡ844bp片段,表达质粒载体为PWR-13,受体菌是E·Coli TAP130。Rb蛋白表达量占细菌总蛋白量的5.04%。利用Rb基因表达产物制备的抗Rb多克隆克体,在视网膜母细胞瘤发病机理的研究中初步应用,获得了重要的实验结果。
The Rb gene expression plasmid was successfully constructed by recombinant DNA technology. The Rb gene fragment was derived from the BglII844bp fragment of Rb cDNA clone P2R4.7. The expression plasmid vector was PWR-13, and the recipient bacteria was E. Coli TAP130. Rb protein expression accounted for 5.04% of the total bacterial protein. The anti-Rb polyclonal Kg made by Rb gene expression product was used preliminarily in the study of the pathogenesis of retinoblastoma, and important experimental results were obtained.