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目的:研究蟒蛇油GC指纹图谱,为蟒蛇油的行业质量控制提供依据。方法:采用气相色谱法,以油酸甲酯为内参照物,采用石英毛细管柱007-225(0.25μm×30 m×0.25 mm),进样口温度280℃,检测器温度280℃,载气流速0.8 mL.min-1,分流比20∶1,进样量为1μL。对24批蟒蛇油进行研究,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A对指纹图谱进行了分析;采用中药色谱指纹图谱相似度评价系统2004B对蟒蛇油与棕榈油、花生油、猪油、普通蛇油的指纹图谱进行比较。结果:24批蟒蛇油样品有9个共有峰,与蟒蛇油GC对照指纹图谱的相似度对比均>0.998,24批蟒蛇油样品的参照峰的丰度范围在0.454～0.493。并采用相似度及参照峰丰度区分了蟒蛇油与棕榈油、花生油、猪油、普通蛇油等油脂。结论:该方法简便、精密性,重复性,稳定性良好,可作为蟒蛇油的质量评价方法。
Objective: To study the GC fingerprints of python oil and provide the basis for the quality control of python oil industry. Methods: Gas chromatography was used with methyl oleate as internal reference. A quartz capillary column 007-225 (0.25 μm × 30 m × 0.25 mm), inlet temperature 280 ℃, detector temperature 280 ℃ and carrier gas flow Speed 0.8 mL.min-1, split ratio 20: 1, injection volume 1μL. Twenty-four batches of python oil were studied, and the fingerprints were analyzed using the 2004A chromatographic fingerprinting similarity evaluation system 2004A promulgated by the Pharmacopoeia Committee. The fingerprint similarity analysis system 2004B was used to evaluate the similarity between python oil and palm oil, peanut oil, lard , The fingerprint of common snake oil is compared. Results: The 24 batches of python oil sample had 9 common peaks, the similarity of which was> 0.998 with the GC fingerprinting of python oil, and the abundance range of reference peaks of 24 batches of python oil samples ranged from 0.454 to 0.493. And the similarity and reference peak abundance distinguish python oil and palm oil, peanut oil, lard, common snake oil and other oils. Conclusion: The method is simple, accurate, reproducible and stable, and can be used as a quality evaluation method for python oil.