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应用原位分子杂交（ＩＳＨ）技术，检测８８例胃癌组织中Ｋ－ｒａｓ、Ｈ－ｒａｓ、Ｃ－ｍｙｃ和肿瘤转移抑制基因ｎｍ２３（Ｈ１）ｍＲＮＡ．其阳性结果分别为７８．４％、７０％、５８％和３８．６％，胃癌癌旁移行区正常粘膜和正常胃粘膜上皮的阳性率分别为１８．２％、１７％、１９．３％、２１．６％和０、０、０、３／５，与肿瘤区相比较，除ｎｍ２３外差异均非常显著（Ｐ＜０．０１）。Ｋ－ｒａｓ、Ｈ－ｒａｓｍＲＮＡ表达在在细胞膜内侧，Ｃ－ｍｙｃｍＲＮＡ主要表达在细胞核内，ｎｍ２３－Ｈ１ｍＲＮＡ表达在胞浆内。４种癌基因表达与组织学类型无关，ｎｍ２３－Ｈ１的表达与胃癌病人有无淋巴结转移有关（Ｐ＜０．０１）。
In situ hybridization (ISH) technique was used to detect K-ras, H-ras, C-myc and metastasis suppressor gene nm23 (H1) mRNA in 88 cases of gastric cancer. The positive results were 78.4%, 70%, 58%, and 38.6%, respectively. The positive rates of normal mucosa and normal gastric epithelium in transitional gastric cancer were 18.2%, 17%, and 19.3%, respectively. , 21.6% and 0, 0, 0, 3/5, compared with the tumor area, except for nm23, the difference was very significant (P <0.01). K-ras and H-ras mRNAs are expressed in the inner membrane of the cell, C-myc mRNA is mainly expressed in the nucleus, and nm23-H1 mRNA is expressed in the cytoplasm. The expression of the 4 oncogenes was not related to the histological type. The expression of nm23-H1 was related to lymph node metastasis in patients with gastric cancer (P<0.01).