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目的:研究半边旗(pteri semipinnataL, PsL)二萜类化合物5F对HO- 8910PM细胞内核因子- κB(nuclear factor kappaB, NF- κB)p65亚基、黏着斑激酶(focal adhesion kinase, FAK)表达及p FAK(phosphorylated FAK)水平的影响,探讨其抗肿瘤侵袭转移的作用机制。方法:MTT法测定5F对细胞增殖的影响; Westernblot分析NF- κB(p65)、FAK蛋白表达及p FAK水平。结果: 12 50、25 00、50 00、100 00 和200 00μmol/L的5F作用HO- 8910PM细胞24h后,细胞增殖抑制率分别为(28 .20±3 .66)%、(30 96±1 83)%、(41 .41±1 .65)%、(60. 67±2. 24)%和(73. 18±1. 24)%,并有一定的剂量效应关系,P< 0 .05 (P 值分别为0 .018、0. 000、0 .000、0 .000和0. 000)。25~100μmol/L的5F作用HO- 8910PM细胞24 h后,NF -κB(p65)蛋白表达水平明显下降;上调FAK蛋白表达,使FAK酪氨酸磷酸化程度降低(p FAK/FAK),P<0. 01(P 值分别为0. 000、0 .001 和0 .000)。结论:5F抗肿瘤侵袭转移与NF- κB(p65)表达以及FAK酪氨酸磷酸化程度降低有关。
OBJECTIVE: To study the effect of diterpenoid 5F from pteri semipinnataL (PsL) on the expression of nuclear factor-kappaB (NF-κB) p65 and focal adhesion kinase (FAK) in HO-8910PM cells, p FAK (phosphorylated FAK) levels, to explore its anti-tumor invasion and metastasis mechanism. Methods: MTT assay was used to determine the effect of 5F on cell proliferation. Western blotting was used to analyze the expression of NF-κB (p65), FAK and p FAK. Results: The inhibitory rates of cell proliferation were (28.20 ± 3.66)% and (30 96 ± 1), respectively, in HO-8910PM cells treated with 12, 25, 50, 50, 100, 100 and 200 μmol / (41.41 ± 1.65)%, (60.67 ± 2.24)% and (73.18 ± 1.24)%, respectively, with a dose-dependent relationship (P <0.05) (P values were 0.018, 0.000, 0.000, 0.000 and 0.000 respectively). The expression of NF-κB p65 protein was significantly decreased in 25- 100μmol / L 5F-treated HO-8910PM cells for 24 h, the expression of FAK protein was increased and the level of tyrosine phosphorylation of FAK was reduced (p FAK / FAK), P <0 01 (P values are 0. 000, 0. 001, and 0 .000, respectively). CONCLUSION: The anti-tumor invasion and metastasis of 5F is related to the decrease of NF-κB (p65) expression and tyrosine phosphorylation of FAK.