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无菌苗的获得是大豆遗传转化中的第一个环节。不同产地的大豆种子可能因其生长环境不同而具有不同程度的带菌率。通过比较不同消毒条件下大豆种子的污染率、发芽率,从而确定一种有效的种子消毒方法。结果表明:不同品种间种子污染率存在显著差异,次氯酸钠液体消毒法对带菌率低的种子消毒有效,经氯气消毒法和二次消毒法处理后的种子污染率明显低于次氯酸钠液体消毒,以二次消毒法对大豆种子的消毒最为彻底;次氯酸钠液体消毒对种子活力影响较大,经氯气消毒和二次消毒的种子活力显著高于次氯酸钠液体消毒。二次消毒法特别适用于带菌率高的种子消毒。
Acquired sterile seed is the first step in soybean genetic transformation. Soybean seeds of different origins may have different degrees of carrier rates due to their different growth environments. By comparing the contamination rate and germination rate of soybean seeds under different disinfection conditions, an effective seed disinfection method was determined. The results showed that there were significant differences in seed pollution rates among different varieties. Sodium hypochlorite liquid disinfection was effective in disinfecting the seed with low germ rate. The chlorine pollution rate after chlorine disinfection and secondary disinfection was significantly lower than that of sodium hypochlorite liquid disinfection. Disinfection method was the most thorough disinfection of soybean seeds; sodium hypochlorite liquid disinfection had a great effect on the seed vigor, the vigor of seed disinfected by chlorine and secondary disinfection was significantly higher than that of sodium hypochlorite liquid disinfection. Secondary disinfection is particularly suitable for seed disinfection with high rates.