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为了优化芸薹属远缘杂交试管苗的快繁培养基,探讨基因型、培养时间等因素对试管苗分化率的影响,实验对甘蓝型油菜与甘蓝以及白菜型油菜与甘蓝两种远缘杂交方式的试管苗,在10种添加了不同浓度的6-BA和NAA的培养基上进行快繁培养。采用SAS软件对培养后第20d和第30d的分化率进行分析处理,结果如下:(1)白菜型油菜与甘蓝5个杂交组合间,以及白菜型油菜与甘蓝、甘蓝型油菜与甘蓝两种杂交类型间的试管苗分化率无显著差异;除甘蓝型油菜与甘蓝杂交的试管苗在培养第30d时的分化率无显著差异外,其它情况下培养基间的诱导分化率差异均达到显著或极显著水平;(2)添加3.0mg.L-16-BA(6-苄基嘌呤)和0.2mg.L-1NAA(萘乙酸)的MS培养基对试管苗的诱导分化效率显著或极显著高于其它快繁培养基;(3)不同培养基培养的试管苗在培养20d和30d时的分化率呈高度正相关,但前20d的日平均分化率显著高于20~30d的日平均分化率。
In order to optimize the fast propagation medium of Brassica hybrids and to explore the effects of genotypes and culture time on the differentiation rate of the tube seedlings, two crosses were conducted between Brassica napus and Brassica campestris and Brassica campestris Way of tube seedlings in 10 kinds of medium supplemented with different concentrations of 6-BA and NAA. SAS software was used to analyze the differentiation rate at the 20th day and the 30th day after culture. The results were as follows: (1) Five crosses between Brassica campestris and Brassica campestris and two crosses between Brassica campestris and Brassica oleracea, Brassica napus and Brassica oleracea There was no significant difference in the rate of in vitro plantlet differentiation among the three types of cultivars except that the differentiation rate of the test-tube seedlings from hybridization between Brassica napus and cabbage did not differ significantly (2) MS medium supplemented with 3.0mg.L-16-BA (6-benzylpurine) and 0.2mg.L-1NAA (naphthaleneacetic acid) significantly or very significantly increased the induction efficiency Other fast propagation medium; (3) There was a highly positive correlation between the differentiation rate of test-tube plantlets cultured in different medium on the 20d and the 30d, but the daily average differentiation rate in the first 20d was significantly higher than the average daily differentiation rate in 20-30d.