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用50天小白鼠64只及124天大白鼠41只,各分4组:Ⅰ组均切除左肾;Ⅱ组均在皮下注射ACTH隔日一次,剂量为每百克体重0.33国际单位,但其中大白鼠在注射前10天先切去左肾,小白鼠不切;Ⅲ组均先切除左肾,10天后注射ACTH,其中小白鼠所用剂量与Ⅱ组同,大白鼠则用15倍于Ⅱ组的剂量;Ⅳ组大、小白鼠均作对照。小白鼠自注射25次开始分批取材,最后一批注射达78次。大白鼠在注射24次后即全部杀死。两批材料均取颈膨大处的脊神经节及同节脊髓,用福尔马林固定,制成石蜡切片。在苏丹黑及PAS染色的切片上观察了神经细胞内呈阳性反应的脂褐色素,计算了在不同处理下含色素细胞所占百分率。并根据色素在细胞内含量的不同等级,比较了不同处理下含各级色素的细胞数量。对所得数据进行了统计分析。结果表明各项实验处理都没有使含色素的神经细胞比对照有所增多,也没有提高细胞内的色素含量。以大白鼠为例,上述四组中含色素的神经细胞所占百分率由Ⅰ到Ⅳ分别为3.31、4.00、3.40及3.70％。各实验组与对照的差异均无统计学意义。以上结果与Sulkin在1960年发表的研究结果不同。他认为去肾及去肾后注射ACTH的大白鼠神经细胞比对照有更多的色素沉积现象。本文在讨论中对比了双方的工作,就Sulkin室实验中的动物只数、研究方法以及他所提出的“应激”反应进行了评述。最后指出,神经细胞内脂褐素的实验性形成问题还远未解决。因此还没有理由完全否认色素沉积是一种内生的衰老变化的指标。
Forty-four mice were divided into four groups: 64 rats in 50 days and 41 rats in 124 days. The animals in each group were divided into four groups: the left kidney was excised in group Ⅰ; the rats in group Ⅱ were injected subcutaneously with ACTH every other day at a dosage of 0.33 IU per hectogram, Rats were cut to the left kidney 10 days prior to injection, the mice were not cut; the first group were all left kidney, 10 days after injection of ACTH, the mice used the same dose and Ⅱ, 15-fold in rats with group Ⅱ Dose; Ⅳ group, white mice were control. The mice were injected in batches 25 times since the injection and the last batch of injection reached 78 times. Rats were killed 24 times after injection. Two batches of materials were taken from the spinal dural enlargement of the spinal ganglion and the same spinal cord, fixed with formalin, made of paraffin sections. The positive reaction of lipofuscin in nerve cells was observed on Sudan black and PAS stained sections and the percentage of pigmented cells under different treatments was calculated. And according to the content of pigment in different levels of different levels compared with different levels of pigment-containing cells. The data were statistically analyzed. The results showed that the experimental treatment did not make the pigment-containing neurons increased compared with the control, did not increase intracellular pigment content. Taking rats as an example, the percentage of neuronal cells containing pigment in the above four groups was 3.31, 4.00, 3.40 and 3.70%, respectively, from Ⅰ to Ⅳ. There was no significant difference between the experimental groups and the control group. The above result is different from the study published by Sulkin in 1960. He believes that rats with renal and renal desease injection of ACTH rat neuronal cells have more pigmentation than the control. This article compares the work of both parties during the discussion, and comments on the number of animals in the Sulkin chamber experiment, the research methods, and the “stress” response he proposed. Finally pointed out that the experimental formation of lipofuscin neurons is far from resolved. There is therefore no reason to completely deny that pigmentation is an endogenous indicator of aging.