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目的:研究松果体和褪黑激素(Mel)是否通过下丘脑影响腹腔巨噬细胞功能.方法:松果体切除术;腹腔巨噬细胞化学发光测定;下丘脑地诺前列酮放射免疫测定;下丘脑注射Mel.结果:松果体切除后腹腔巨噬细胞化学发光值降低,下丘脑地诺前列酮含量升高,16:00 ip Mel(10 μg kg~(-1)d~(-1)×7d)可使其恢复,并升高正常大鼠腹腔巨噬细胞化学发光值,降低其下丘脑地诺前列酮含量.腹腔巨噬细胞化学发光值与下丘脑地诺前列酮含量的变化存在负相关(相关系数,r=-0.78,P<0.01).于下丘脑注射Mel 2μg,能提高正常大鼠和松果体切除大鼠腹腔巨噬细胞化学发光值.结论:下丘脑是松果体Mel影响腹腔巨噬细胞功能的主要作用部位之一.
Objective: To study whether the pineal and melatonin affect the function of peritoneal macrophages through the hypothalamus.Methods: Pineal resection, chemiluminescence of peritoneal macrophages, denudinone radioimmunoassay, Hypothalamic Melodipine injection.Results: The chemiluminescence of peritoneal macrophages decreased after pineal excision, and the content of dinoprostone in hypothalamus increased 16:00 ip Mel (10 μg kg -1 d -1 ) × 7d), which could restore the chemiluminescence of normal rat peritoneal macrophages and decrease the content of dinoprostone in hypothalamus.Changes of chemiluminescence of peritoneal macrophages and hypothalamus dinoprostone content (R = -0.78, P <0.01) .The injection of Mel 2 μg into the hypothalamus can improve the chemiluminescence of peritoneal macrophages in normal rats and pineal glands.Conclusion: The hypothalamus is loose Mel affects the function of peritoneal macrophages one of the main role.