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目的:探讨纳米二氧化锰(nano-MnO2)对大鼠腹侧中脑的损伤作用。方法:大鼠在脑立体定位下,实验组脑内注射nano-MnO2,对照组脑内注射生理盐水(NS),各组分别于注射1周后用免疫组织化学方法检测酪氨酸羟化酶(tyrosine hydroxylase,TH)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)及诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达变化。结果:免疫组织化学染色结果显示,实验组大鼠注射1周后,腹侧中脑损毁侧与对侧相比,TH阳性细胞明显减少(P<0.05),GFAP及iNOS阳性细胞明显增多(P<0.05);对照组大鼠注射1周后,腹侧中脑损毁侧与对侧相比,TH、GFAP和iNOS免疫反应阳性细胞均无明显变化(P>0.05)。结论:脑内注射nano-MnO2能引起大鼠中脑多巴胺能神经元的破坏,GFAP和iNOS的表达增加。
Objective: To investigate the effect of nano-manganese dioxide (Mn-MnO2) on the ventral midbrain of rats. Methods: Rats were injected with nano-MnO2 intracerebroventricularly (NS) in the experimental group under stereotaxic condition. The rats in each group were injected with saline (NS) for 1 week, respectively. Immunohistochemistry was used to detect tyrosine hydroxylase tyrosine hydroxylase (TH), glial fibrillary acidic protein (GFAP), and inducible nitric oxide synthase (iNOS) Results: Immunohistochemical staining showed that TH-positive cells were significantly decreased (P <0.05) and GFAP and iNOS positive cells were significantly increased in the ventral midbrain lesion side compared with the contralateral side (P <0.05). Compared with the contralateral side, there were no significant changes in the immunoreactive TH, GFAP and iNOS positive cells in the control group after 1 week of injection (P> 0.05). CONCLUSION: Nano-MnO2 injected into the brain can cause the destruction of dopaminergic neurons in the midbrain of rats, and the expression of GFAP and iNOS increased.