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苯酚是一种重要的化工原料并广泛存在于工业废水中,随着各国对苯酚生物毒性的认识,排放标准日益提高。生物法作为一种高效、低成本、不易二次污染的方法常用于含酚的废水处理。但是可降解苯酚的微生物筛选却是一个复杂繁琐的过程。衰减全反射傅里叶红外光谱(attenuated total reflection Fourier transform infrared,ATR-FTIR)技术是一种高效、快捷、高指纹特性的物理检测技术,主成分分析联用最小偏二乘法(principal component analysis-partial least squares,PCA-PLS)是一种有效提取特征指纹峰并建立模型的方法,该实验联合ATR-FTIR检测技术和PCA-PLS统计方法建立苯酚浓度与吸光度模型,可以快速检测固体培养基中底物浓度。实验建立模型判定系数可以达到99.5%,预测集的判定系数可以到达99.4%,说明模型具有较高的拟合性和推广性。通过模型可以预测出菌株降解后固体培养基底物浓度,筛选出可降解苯酚功能微生物,传统的液体培养并采用气相色谱检测残留苯酚浓度筛选出的结果与ATR-FTIR方法筛选出结果进行对比发现,得到相同的筛选结果。结果表明ATR-FTIR联合PCA-PLS建立高拟合度模型,可以快速检测固体培养基底物浓度,从而达到快速筛选可降解苯酚菌的目的,这种方法可以应用到其他有特征指纹峰的底物中,ATR-FTIR是一种可以广泛应用到功能微生物筛选的快速检测方法。
Phenol is an important chemical raw material and widely exists in industrial wastewater. With the cognition of the biological toxicity of phenol in various countries, the emission standard is increasing day by day. Biological method as a high-efficiency, low-cost, easy to secondary pollution methods commonly used in phenol wastewater treatment. However, microbial biodegradation of phenol screening is a complex and cumbersome process. Attenuated total reflection Fourier transform infrared (ATR-FTIR) technique is a kind of physical detection technique with high efficiency, fastness and high fingerprint. Principal component analysis combined with principal component analysis- Partial Least Squares (PCA-PLS) is a method of extracting characteristic fingerprint peaks and establishing a model. The experiment combined with ATR-FTIR detection and PCA-PLS statistical methods to establish phenol concentration and absorbance model, Substrate concentration. The experimentally established model can reach 99.5% of the coefficient of determination, and the coefficient of determination of the prediction set can reach 99.4%, indicating that the model has a high fitting and generalization. Through the model, we can predict the substrate concentration of the solid culture medium after degrading the strain, screened the biodegradable phenol functional microorganisms, the traditional liquid culture and the residual phenol concentration detected by gas chromatography were compared with the results of ATR-FTIR screening method. Get the same screening results. The results showed that ATR-FTIR combined with PCA-PLS to establish a high fitness model can quickly detect the solid substrate concentration, so as to achieve the purpose of rapid screening of degradable phenol bacteria. This method can be applied to other substrates with characteristic fingerprint peaks , ATR-FTIR is a rapid detection method that can be widely applied to functional microbial screening.