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[目的]评估槐果碱的体外抗弓形虫作用.[方法]采用CCK-8法测定槐果碱对HeLa细胞的毒性和对弓形虫选择性作用的效果,光学显微镜下观察细胞形态变化,应用台盼蓝拒染法检测活细胞百分比.[结果]槐果碱浓度为500μmol/L时,对HeLa细胞有明显的抑制作用;槐果碱体外抗弓形虫作用的选择性指数为0.96,效果低于乙胺嘧啶(1.77),但优于螺旋霉素(0.73);给予200μmol/L槐果碱治疗24 h后,HeLa细胞破损程度明显降低,细胞内外速殖子数量显著减少,且在10~200μmol/L浓度范围内时活细胞比例随给药浓度的增加而明显上升.[结论]槐果碱在体外表现出良好的抗弓形虫活性,且对弓形虫感染的HeLa细胞具有一定程度的保护作用.
[Objective] To evaluate the effect of sophocarpine on Toxoplasma gondii in vitro. [Methods] The cytotoxicity of sophocarpine on HeLa cells and the selective effect on Toxoplasma gondii were determined by CCK-8 assay. The morphological changes of cells were observed under light microscope. Trypan blue exclusion method was used to detect the percentage of viable cells. [Results] When the concentration of sophocarpine was 500 μmol / L, HeLa cells were significantly inhibited. The selective index of anti-Toxoplasma activity of sophocarine in vitro was 0.96 with low effect (1.77), but better than spiramycin (0.73). After treated with 200 μmol / L sophocarpine for 24 h, the degree of damage of HeLa cells was significantly reduced and the number of tachyzoites in and out of the cells was significantly reduced. The proportion of viable cells in the concentration range of 200μmol / L increased significantly with the increase of the drug concentration. [Conclusion] Sophocarpine showed good activity against Toxoplasma gondii in vitro and had some degree of protection against Toxoplasma gondii infection of HeLa cells effect.