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50只雄性昆明小鼠适应性饲养1周后随机分为5组,分别为空白对照组、模型组及金线莲多糖(ARP)低、中、高剂量组,每组10只;第1~3天,除空白对照组注射生理盐水外,其他各组腹腔注射环磷酰胺(CTX)80mg/kg,连续3d;第4~10天,空白组和模型组灌服蒸馏水,其他组分别灌服100,200,400mg/kg的ARP。末次给药24h后,采集脾脏,qRT-PCR检测试验小鼠脾脏细胞因子(IL-2、IFN-γ、IL-4、IL-6)及转录因子(T-bet、GATA-3)mRNA表达。结果显示:与空白对照组相比,模型组IL-2、GATA-3 mRNA表达量降低,IL-4、IL-6、IFN-γ、T-bet mRNA表达量升高;与模型组相比,ARP高剂量组小鼠IL-4mRNA相对表达量降低(P<0.05),中、高剂量组IL-6、IFN-γmRNA相对表达量降低(P<0.05),ARP组T-bet的相对表达量降低(P<0.05),高剂量组GATA-3的相对表达量升高(P<0.05)。结果表明:ARP通过上调或下调小鼠脾脏细胞因子与转录因子的表达,调节免疫抑制小鼠的免疫功能。
Fifty male Kunming mice were randomly divided into five groups after one week of adaptive feeding, which were blank control group, model group and low, medium and high dose of A. sinense (ARP) After 3 days, all the rats in the blank control group were injected intraperitoneally with CTX 80mg / kg for 3 days. The rats in the blank group and the model group were given distilled water, 100, 200, 400 mg / kg ARP. Twenty-four hours after the last administration, the spleen was collected and the expression of cytokines (IL-2, IFN-γ, IL-4 and IL-6) and transcription factor T-bet (GATA-3) . The results showed that compared with the blank control group, the expression of IL-2 and GATA-3 mRNA in model group decreased and the expression of IL-4, IL-6, IFN-γ and T-bet mRNA increased (P <0.05). The relative expression of IL-6 and IFN-γmRNA in medium and high dose groups decreased (P <0.05), and the relative expression of T-bet in ARP group (P <0.05), and the relative expression of GATA-3 in high dose group increased (P <0.05). The results showed that ARP can regulate the immune function of immunosuppressed mice by up-regulating or down-regulating the expression of cytokines and transcription factors in spleen of mice.