SSR标记鉴定双低甘蓝型油菜杂交种纯度

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随着分子生物学的发展,分子标记技术已广泛应用于各类作物的纯度鉴定。本实验室前人筛选了3对在几种双低甘蓝型油菜杂交种双亲中呈共显性且多态性良好的SSR标记引物(SSR83,SSR660,SSR740),但未做相应的田间鉴定比较分析,本实验旨在通过将田间鉴定结果和SSR标记鉴定结果进行比较,判断上述3个标记用于纯度鉴定的准确性。利用本实验室开发的3对SSR标记引物,鉴定12份杂交油菜种子样品纯度,统计父本率、不育株率、可育株率及纯度。同时,每份样品进行田间种植。花期进行育性调查,统计可育植株的比
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